|Year : 2014 | Volume
| Issue : 5 | Page : 78-92
|Biomimetic ceramics for periodontal regeneration in infrabony defects: A systematic review
Jasuma Jagdish Rai1, Thanveer Kalantharakath2
1 Department of Periodontics, K. M. Shah Dental College and Hospital, Sumadeep Vidyapeeth, Gujarat, India
2 Department of Public Health Dentistry, K. M. Shah Dental College and Hospital, Sumadeep Vidyapeeth, Gujarat, India
|Date of Web Publication||4-Dec-2014|
Department of Public Health Dentistry, K. M. Shah Dental College and Hospital, Sumadeep Vidyapeeth, Village Piparia, Waghodia Takuka, Vadodara 391 760, Gujarat
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Biomimetic materials are widely used in the treatment of osseous defects as an alternative to autogenous bone graft. The aim of this article was to review the literature and compare the quality of published articles on biomimetic ceramic material used for periodontal regeneration in the treatment of infrabony defects and to discuss the future direction of research. The bibliographic databases PubMed, Ebsco, and Google Scholar were searched from January 2000 to March 2014 for randomized control trials in which biomimetic ceramic graft material was compared with open flap debridement or in combination with any other regenerative material. To avoid the variability of the search terms, the thesaurus Mesh was used. The primary outcome variable assessed was clinical attachment level (CAL). The screening of eligible studies, assessment of the methodological quality of the trials, and data extraction were performed by two observers independently. Twenty-six articles were identified and included in this systematic review. The primary outcome was CAL. Out of the 26 studies, 24 showed more than 2 mm of CAL gain. The difference in CAL change between test and control groups varied from 1.2 mm to 5.88 mm with respect to different biomaterials/biomimetic materials, which was clinically and statistically significant. Meta-analysis was not done due to heterogeneity in results between studies. Overall, biomaterials were found to be more effective than open flap debridement in improving the attachment levels in intraosseous defects. Future research should aim at increasing the osteoinductive capacity of these biomimetic graft materials.
Keywords: Biomaterials, biomimetic materials, bone grafts, infrabony defects, systematic review
|How to cite this article:|
Rai JJ, Kalantharakath T. Biomimetic ceramics for periodontal regeneration in infrabony defects: A systematic review. J Int Soc Prevent Communit Dent 2014;4, Suppl S2:78-92
|How to cite this URL:|
Rai JJ, Kalantharakath T. Biomimetic ceramics for periodontal regeneration in infrabony defects: A systematic review. J Int Soc Prevent Communit Dent [serial online] 2014 [cited 2020 Aug 11];4, Suppl S2:78-92. Available from: http://www.jispcd.org/text.asp?2014/4/5/78/146207
| Introduction|| |
Bone grafting in dentistry is indicated in several surgical situations such as treatment of bone defects, facial clefts, re-construction of alveolar ridge, socket preservation, sinus lift, treatment of peri-implantitis, and endodontic surgeries. Autogenous grafts are used to enhance regeneration and healing of the defect site. Cancellous autograft is considered the gold standard for bone grafts, but it has its own limitations like availability, morbidity, and infection of the surgical site. This has initiated the development of several bone graft alternatives called biomaterials. While earlier the materials were designed to be bioinert, scientists have shifted their focus toward designing bioactive materials that integrate biological molecules, cells, and regenerate tissue,  which can offer novel methods of generating biological solutions for design and synthesis of composite materials such as bone, cartilage, cementum, periodontal ligament, skin, enamel, and dentin, re-construction of alveolar ridge, temporomandibular joint, and other joint prosthesis, new polymers for guided tissue regeneration in the treatment of bone and connective tissue defects, and growth factors to induce bone healing and developing dental and facial implants. The aim of this review is to determine and compare the quality of research articles published in the field of periodontal regeneration using biomimetic ceramic graft material with open flap debridement (OFD) or in combination with any other regenerative material in the treatment of infrabony defects.
| History|| |
Nature has always served as a model for inspiration, as evident in the long and rich heritage of human artifacts and technology.  In 1960, the process of copying from nature was regarded as a scientific discipline. Scientists have coined various names for the specific use of nature as inspiration in design (bionics, biomimetics, bio-inspiration, and biognosis). In 1969, Otto H. Schmitt, a biomedical engineer, coined the term "biomimetics" which describes an electronic feedback circuit he designed to function in a similar way to the neural networks.  "Biomimetics" has a Greek origin, with the words "bios" meaning life and "mimesis" meaning to copy. It is a new field that implements concepts and principles from nature in creating new materials, devices, and systems. 
The concept of biomimetics is vast and biomimicry finds its applications in several fields starting from aeronautics to earth sciences to medicine to zoology. In the field of medicine, biomimicry has been reported since the days of Emperor Nero in the first century AD. Nero, who was short-sighted, used an emerald to magnify things for a better vision; he got this idea from dew drops which act as a magnifying lens depending on the shape.  Today we have pacemakers that mimic the impulses of the sinoatrial (SA) node of the heart. Tiny serrations on the mosquito's proboscis have inspired a team of Japanese scientists to make relatively painless hypodermic needle edges. 
| Search strategy|| |
This article is an attempt to review the literature on biomimetic ceramic material used for periodontal regeneration in the treatment of infrabony defects and to discuss future direction of research. The historical and human histological data were extracted after a thorough review of the literature. A systematic search for literature reports was carried out to identify relevant studies (randomized control trials only) by using the keywords "biomaterials in treatment of infrabony defects" and "biomimetics materials in treatment of infrabony defects," and each biomimetic ceramic graft material used for treatment in infrabony defects was individually searched. The research articles were searched from 1 Jan 2000 to 30 March 2014 in PubMed, Ebsco database, and Google Scholar search engine. The hand search was limited to six periodontal journals during the years 2000 through 2014. In addition, the reference lists of all relevant articles were searched; initial screening of titles and abstract was performed and only full-text articles were included [Figure 1].
Articles on the regenerative outcome of synthetic ceramic bone replacement materials in the treatment of human infrabony defects were considered for inclusion in this review. The follow-up duration of the studies were more than 6 months and the primary outcome variable assessed was clinical attachment level (CAL). Other outcome variables assessed were probing pocket depth (PPD) and/or radiographic parameters and/or surgical re-entry measurements. The articles were restricted to English language. Exclusion criteria included non-randomized observational studies, publications providing summary statistics without variance estimation or data to permit computation, and studies without a bone replacement graft intervention alone.
| Quality assessment|| |
The methodological quality for the included studies was assessed with a predetermined appraisal form focusing on the following issues: Bibliographic details , the method of randomization and blinding of patients, therapist and examiners, characteristics of the study population, frequency and course of the interventions, baseline and outcome measures, and completeness of follow-up. To achieve a discriminative objective, two reviewers (TK and JJR) independently assessed the quality of each study. Disagreements on validity assessment were resolved by consensus and discussion.
The ideal biomaterial must be compatible, resorbable, and porous to facilitate rapid vascularization and progressive replacement of newly formed tissue.  The majority of biomimetic materials used in regenerative medicine aim to replicate the porous architecture of cancellous bone. Research shows that the requisite pore size for ingrowth of bone is 150-500 μm and to stimulate fibrovascular growth, the pore diameter should be more than 100 μm. ,
According to the European Society for Biomaterials, a biomaterial is a material intended to interface with biological systems to evaluate, treat, augment, or replace any tissue, organ, or function of the body. 
| Types of Biomaterials|| |
- Ceramics- bioinert ceramics, bioactive ceramics, biodegradable ceramics
- Polymers- bioinert polymers, bioresorbable polymers
- Metal- 316L stainless steel, commercially pure titanium alloys and titanium alloys, cobalt-chromium alloys
According to the activity of biomaterials, they could be classified as: 
- Osteoconductive biomaterials which provide scaffold or framework that supports bone growth and encourages the ingrowth of surrounding bone,
- Osteoinductive biomaterials comprising combination of growth regulatory molecules with carriers, and
- Osteogenic biomaterials which contain osteocompetent cells.
Only synthetic biomaterials/biomimetics (of the first category, i.e. ceramics) were taken into consideration for discussion in this systematic review.
Ceramics are crystalline, inorganic, non-metallic minerals that are held together by ionic bonds and usually densified by sintering. 
| Biomaterials Classification|| |
When a synthetic material is placed within the human body, the tissue reacts toward the implant in different ways depending on the material type. The mechanism of tissue interaction depends on the response of the tissue to the implant surface. In general, there are three terms by which a biomaterial may be described or classified into representing the tissues responses. These are bioinert, bioresorbable, and bioactive.
The term bioinert refers to any material which has minimal interaction with its surrounding tissue when placed in the human body, e.g. stainless steel, titanium, alumina, partially stabilized zirconia, and ultra-high-molecular-weight polyethylene. Generally, a fibrous capsule might form around bioinert implants; hence, its bio-functionality relies on tissue integration through the implant.
Bioactive refers to a material which upon being placed within the human body, interacts with the surrounding bone and, in some cases, even soft tissue. This occurs through a time-dependent kinetic modification of the surface that is triggered by its implantation within the living bone. An ion-exchange reaction between the bioactive implant and surrounding body fluids results in the formation of a biologically active carbonate apatite (CHAP) layer on the implant that is chemically and crystallographically equivalent to the mineral phase in bone. Examples of these materials are synthetic hydroxyapatite (HA) and bioglass.
Bioresorbable refers to a material which starts to dissolve upon placement within the human body and is slowly replaced by advancing tissue (such as bone). Common examples of bioresorbable materials are tricalcium phosphate (TCP), polylactic-polyglycolic acid copolymers, and gypsum. 
Ceramics used in periodontal regeneration are:
- Calcium sulfate (CS)
- Calcium phosphate
Biphasic calcium phosphate (BCP)
Tricalcium phosphate (TCP)
Calcium phosphate cement (CPC)
- Bioactive glass (BG)
- Ion-substituted bioceramics
| Results|| |
The search resulted in the identification of 259 studies. Independent initial screening of the titles and abstracts by two reviewers (TK and JJR) resulted in further consideration of 45 randomized controlled trials for possible inclusion [Figure 1]. Of these studies, 26 articles met the defined inclusion criteria, i.e. 2 studies on calcium sulfate, 4 studies on HA, 6 studies on β-TCP, 6 studies on BCP, 2 studies on CPC, 5 studies on BG, and 1 study on composite grafts, were reviewed in this systematic review [Table 1]. All articles included have low to moderate risk of bias.
|Table 1: Characteristics of RCT studies comparing ceramic biomaterials (biomimetic materials) in treatment of infrabony defects |
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CAL has been taken as a primary outcome variable as it gives an approximate clinical measurement of loss or gain of connective tissue attachment from the root surface.  All the studies included showed a positive effect in relation to CAL and PPD, when compared to OFD. The difference in CAL change between test and control groups varied from 1.2 mm to 5.88 mm with respect to different biomaterials/biomimetic agents, which was clinically and statistically highly significant [Figure 2]. Only two studies showed less than 2 mm of CAL gain, which was in relation to bioactive glass and TCP [Table 1].
Each ceramic biomimetic graft material is described below.
|Figure 2: Plot of some of the randomized control trials (RCTs) comparing biomimetic ceramic materials in the treatment of infrabony defects, which were published between 2000 and 2014. The red square indicates the average clinical attachment level (CAL) reported|
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Calcium sulfate (CaSO 4 ) got its name plaster of Paris after a small village just north to Paris. It was used to fill bone defects caused by tuberculosis. In 1892, Dressman first reported the use of calcium sulfate in human skeletal defects to fill voids in long bones caused by tuberculous osteomyelitis.  It is one of the first synthetic bone grafts used as a replacement for autograft. 
After being placed into the bone defect, calcium sulfate undergoes degradation to calcium and sulfate ions. Calcium ions combine with phosphate ions from body fluids to form calcium phosphate, which provides an osteoconductive surface that stimulates the recruitment of osteoblasts and development of new bone in the defect. As calcium sulfate undergoes degradation in the body, there is a local decrease in pH. This pH drop results in demineralization of defect walls, thus releasing bone growth factors which stimulate the formation and development of new bone. This newly deposited material is mainly carbonated HA which is similar to apatite that is naturally present in bone. The graft material gets resorbed within 6 weeks, which is much faster than that of HA and TCP. Its degradation exceeds the rate of new bone growth into the defect; hence, to overcome this limitation, it can be used along with other graft materials.  Calcium sulfate is reabsorbed by a process of dissolution over a period of 5-7 weeks  [Table 2].
In 1997, Pecora  concluded that it works as a barrier membrane by excluding the growth of connective tissue and allowing bone regeneration. Calcium sulfate was also observed to possess angiogenic properties. In 2002, Strocchi et al.  reported that more blood vessels grew into the defects filled with calcium sulfate than those filled with autograft. It can effectively be used as a drug delivery vehicle. Several drugs like Tobramycin (Beardmore et al. in 2005)  and Simvastatin (Nyan et al. in 2007)  have been delivered locally through calcium sulfate. Budhiraja showed parallel results when Demineralization Freeze Dried Bone Allograft (DFBBA) and collagen membrane was compared with DFDBA and Calcium Sulphate (CS) indicating that CS is effective as a collagen membrane as a barrier material. 
It is available in combination with HA or demineralized bone matrix, or as a "binder" type of material designed to be mixed with various alloplasts, allografts, or autografts to improve handling and prevent particle migration [Table 1]. Examples: Calcigen, Capset, Calmatrix, Surgiplaster
Use of calcium phosphate ceramics was first proposed by Albee and Morrision in 1920 for biomedical applications.  HA is a naturally occurring mineral form of calcium phosphate that constitutes up to 70% of the dry weight of bone. HA was first identified as the mineral component of bone by De Jong in 1928. 
Two forms of HA are available: Natural and synthetic. Synthetic HA may be porous and non-porous. Non-porous HA does not resorb; the porous synthetic form of HA is osteoconductive and has a crystalline structure similar to the HA in bone. Porous synthesized HA is slower to resorb than the endogenous form and may stay at the site of implantation for many years  [Table 1]. In porous granular form, it can be used alone or with bone graft to fill voids. It is successfully used to coat metal implants to enhance their osseointegration. 
Microcrystalline, non-ceramic HA
Manufactured using a low-temperature precipitation process, micro-crystalline, non-ceramic HA is a readily resorbable source of bioactive calcium phosphate. By avoiding high-temperature processes, these materials do not become ceramics and maintain a chemistry that is very similar to biological apatites. The crystals are not resorbed by cell-mediated processes; rather they are dissolved into solution, providing a ready source of calcium and phosphate as well as a structural lattice which can support early bone formation. 
Examples : OsteoGen non-ceramic, microcrystalline HA powder
HA resorbs by cellular resorption during bone remodeling. Residual HA and bone growth ranges are 0-55% and 18-56%, respectively. HA coating is increasingly resorbed with time from implantation and is nearly completed at 8 years. The only demographic factor that influences the amount of bone ongrowth is age, with younger patients having higher bone ongrowth percentages than older patients. This may relate to greater initial bone stock in younger people, but can also be explained by the fact that in older patients, the resorptive component of the remodeling process is more prominent. 
In 2011, in a histological study, Checchi et al. found the percentage of new mature bone to be 49 ± 28% in the biopsy indicating the bone-forming ability and the percentage of osteoid tissue and remaining material to be 14 ± 7% indicating remodeling capacity after 6 months. It was concluded that the graft degrades in a non-homogenous manner. 
In 2013, Horvath et al.  in a histological study showed healing predominately characterized by epithelial downgrowth, limited formation of new cementum and connective tissue fibers with bone regeneration occurred in three out of the six biopsies. Complete resorption of the nano-HA was found in four out of the six biopsies. A few remnants of the graft particles were seen either surrounded by newly formed mineralized tissue or encapsulated in connective tissue in two out of the six biopsies.  HA shows better results compared to OFD (Kasaj et al.) and when used in combination with other regenerative materials [Table 1].
Tricalcium phosphate (TCP)
TCP is a bioceramic that is resorbed faster than synthetic HA, but is not as strong. It exists in alpha and beta crystal forms. β-TCP has been effectively used in dental procedures and as a component of bioresorbable screws since 1981. The material has value as a bone graft extender and mineral source. The graft particles are composed of a highly porous matrix with 100-300 μm pore size. Osteoconduction is facilitated by the porous nature of the particles, with bone growth said to occur within and throughout the porous matrix. The particles are eventually resorbed and replaced by host bone in 9-12 months [Table 2].
β-TCP particles are embedded in the connective tissue, whereas the formation of a mineralized bone-like or cementum-like tissue around the particles was only occasionally observed. Stavropoulos et al. concluded in their study that the present data indicates that treatment of intrabony periodontal defects with β-TCP may result in considerable clinical improvement in CAL gain and PD reduction, but β-TCP does not seem to enhance the regeneration of cementum, periodontal ligament and bone. 
Porous β-TCP may be used as a vehicle for the delivery of drugs or biological agents. Recently, an enhanced version of β-TCP containing recombinant platelet-derived growth factor (rhPDGF-BB) has been introduced. Conceptually, this product combines the benefits of an osteoconductive scaffold with a mitogenic growth factor, allowing for precisely tailored dosage and localized delivery of a compound with proven wound healing and periodontal regenerative benefits. 
In 2008, Ridhway conducted a histological study to evaluate rhPDGF-BB in combination with β-TCP for the treatment of human intraosseous periodontal defects. After 6 months of minimum healing, the tooth was removed en bloc. New bone, new cementum, and new periodontal ligament had regenerated coronal to the notch placed on the root surface. New cementum formed on dentin and on old cementum. Connective tissue arrangement occurred in both parallel and perpendicular arrangements with majority of fibers aligned parallel to the root surface. Variable amounts of β-TCP particles were seen with minimal inflammatory infiltrate. Minimal amounts of newly formed bone were observed in contact with β-TCP.  Nevins et al. (2005)  and Jayakumar et al.  conducted a study in which they used rhPDGF-BB/β-TCP and found that implantation in intraosseous periodontal defects was safe, well tolerated, and resulted in clinically and statistically significant improvement in bone formation parameters as well as soft tissue outcomes  [Table 1].
Examples: Bioresorb β-TCP, CeraSorb, Vitoss porous β-TCP ceramic, GEM-21S (porous β-TCP/rhPDGF-BB)
Biphasic calcium phosphate
HA and β-TCP may be combined in different ratios into a single product known as BCP. BCP is engineered to combine the advantages of both HA and β-TCP. Straumann bone ceramic (SBC), has 40% β-TCP and 60% HA (higher the ratio of TCP, greater the resorbability).  The rapid dissolution of TCP provides calcium and phosphate ions as well as space for bone formation, while the slower resorbing HA maintains the scaffold until sufficient bone ingrowth has occurred , [Table 2]. The open structure of BCP with interconnected macropores (>100 μm) promotes vascular infiltration, nutritional transport, and cell colonization, while a 3-dimensional, microporous architecture (<10 μm) creates a favorable environment for adsorption of macromolecules and cell attachment.
The replacement of TCP by bone does not occur in an equal manner. There is less bone volume produced than the volume of TCP resorbed.  Jensen et al.  compared the percentage of new bone formation by SBC with that of autogenous bone, HA, and β-TCP separately over a 24-week period. They found that SBC was better than HA alone, but formed less bone than β-TCP and autogenous bone [Table 1].
Examples: OsSatura BCP, SBC
Calcium phosphate cement
The lack of adaptability of calcium phosphate ceramics was resolved by Brown and Chow in 1985 when they developed CPC.
CPC formulations are classified with respect to their end products. Current CPCs can be divided into two categories: (i) apatitic and (ii) brushitic cements.
This cement is a mixture of calcium phosphate powders which, on reacting with an aqueous phase, produce new calcium phosphate compounds. The consistency of the cement progresses from paste-like to solid structure by entanglement of the setting product. This enables the cement to be molded, to adapt to bone defect borders, and permits the development of injectable preparation for minimally invasive surgery. These cements are biocompatible, degradable, and osteoconductive,  and management of human intrabony defects with the use of CPC shows improved clinical outcome compared to OFD  [Table 1].
Because of their excellent biocompatibility and non-exothermic behavior, it is possible to incorporate organic molecules in these cements, making them potential vector materials for the therapeutic agent delivery.
In two studies, novel amorphous CPC (Biobon) was implanted in human patients for the first time. After 2-12 months, 10 biopsies were obtained during the second surgical procedure. In all specimens, partial replacement by new bone was observed, while residues of the cement were still visible. Under calcified sections extensive bone formation in immediate contact with the cement without fibrous interface was observed. Polynucleated cells and superficial lacunae were indicative of resorptive activity; inflammatory quotient was absent. The new bone displayed regular trabecular and osteonatal patterns. 
Calcium phosphate is osteoconductive and undergoes gradual remodeling over time, mainly through a cell-mediated surface process involving osteoclasts and osteoblasts. CPC resorbs over a period of 24 months [Table 2].
Bioactive glass was discovered by Dr. L. Hench in 1969. The initial evidence of direct bond between the product and bone was given by Hench et al. in 1972. The unique feature of bioglass that differentiates it from other bioceramic alloplastic materials is its bioactivity. A bioactive material is defined as a material that elicits a specific response at the interface of the material, which results in formation of a bond between the tissue and that material.  When bioactive ceramics are implanted, they undergo surface modification, upon exposure to interstitial fluids, the pH of the local environment increases and approaches 10. A silicon-rich layer is formed on the bioactive ceramic surface, and then on top of this, a calcium phosphate-rich layer is formed from the calcium and phosphorous of the bioactive ceramic and that of the body fluids. The calcium phosphate layer is an active hydroxyl CHAP layer that serves as the bonding surface and is chemically and structurally equivalent to the mineral composition of bone. This reaction layer develops within minutes/hours of implantation (Hench et al. 1990),  and then osteogenic cells and collagen fibers from the host surgical site colonize the surface of the bioactive ceramic particles (bioactivity), becoming incorporated into the silica gel layer and eventually producing bone , [Figure 3]. Bioacive glass (BG) was approved by US Food and Drug Administration (FDA) in 1996 for use as a bone graft.
|Figure 3: Sequence of reactions involved in forming a bond between bioactive glass and bone. There are 11 stages in the process of complete bonding of bioactive glass to bone. Stages 1-5 show the chemical response and stages 6-11 show the biological response between BG and bone.[58,59]|
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In 2000, Nevins et al.  studied the healing of intrabony defects around five teeth grafted with BG. Healing was evaluated by clinical, radiographic measurements and histological analysis. After 6 months of surgery, there was 2.7 mm probing depth reduction and clinical attachment gain of 2.2 mm. Histological analysis showed one case healing by new cementum and new connective tissue formation and the rest of the cases healing by long junctional epithelium. Bone formation was limited to the most apical borders of the defect and the particles were found to be biocompatible with minimal inflammatory infiltrate. The mechanism of action is through osteoconduction. Larger particles may take years [Table 2]. Bioactive glass has been used extensively in the treatment of periodontal defects. 
In 2012, Sohrabi et al. found in their meta-analysis that treatment of intrabony defects with BG imparts a significant improvement in both PD and CAL, compared to both active controls and OFD. When BG and Enamel matrix derivative (EMD) were clinically compared, it might be interpreted that BG is equally effective as EMDs in the treatment of intraosseous defects. Bioglass materials have been used extensively in periodontal regeneration with good results. The primary indication of these materials is for the repair of small, localized infrabony defects.
Examples : Perioglass, Novabon Putty, Biogran
| Future research|| |
Ion substitution refers to the process wherein an ion within a substance is exchanged for another ion with the same (i.e. positive or negative) charge. Biomineralization combined with ion substitution is advantageous due to ion-substituted calcium phosphate coatings having a high similarity to the natural mineral of bone, which have beneficial effects on the anchoring of an implant to host tissue and bone regeneration.  There is increasing interest in developing biomaterials with carefully selected impurities to improve bioactivity. By substituting ions such as silicate, carbonate, magnesium, fluoride, and strontium, biomaterials with various compositions have emerged.
Silicon has been found in greatest concentration in immature bone. It has been proposed that silicon is involved in the initiation of calcification through an effect on the pre-osseous matrix.  Synthetic HA that includes trace levels of Si in its structure demonstrates markedly increased biological performance in comparison to HA.  The improvement in biological performance can be attributed to Si-induced changes in the material properties and also to the direct effects of Si on the physiological processes of the bone and connective tissue systems. Si substitution promotes biological activity by the transformation of the material surface to a biologically equivalent HA by increasing the solubility of the material, by generating a more electronegative surface, and by creating a finer microstructure. Release of Si complexes to the extracellular media and the presence of Si at the material surface may induce additional dose-dependent stimulatory effects on the cells of bone and cartilage tissue systems. 
Silicate-substituted calcium phosphate (Si-CaP) is a bioceramic in which phosphate ions have been substituted with silicate ions at a level of 0.8 wt%. Small amount of silicate seems to promote rapid apposition of immature bone, while 0.8 wt% is the optimal amount of silicate that enhances local bone bioactivity. 
Carbonate (CO3 2− ) is the most abundant (2-8 wt%) anionic substitute, and partially substitutes both in PO4 3 − site and OH − site of calcium phosphate structure. The high reactivity of young bone could be related to the greater presence of carbonate compared with old bone. Carbonated calcium phosphate has shown improved solubility, increased collagen deposition and reabsorption compared with calcium phosphate.
Fluoride exists in bone and teeth of vertebrate bodies. It was reported that the substitution of fluoride for OH sites and formation of fluoride-substituted HA enhanced the acid resistance and the mechanical properties of HA bioceramics  and induced better biological response.  The superior acid resistance and the mechanical property make fluoride-substituted HA a beneficial coating on the dental implant.
Strontium is chemically and physically closely related to calcium. So, it is easily introduced as a natural substitute of calcium in HA. Strontium has been found to have the effects of increasing bone formation and reducing bone resorption, leading to a gain in bone mass and improved bone mechanical properties in normal animals and humans. 
Magnesium has been found in high concentrations in bone and cartilage tissue during the initial phases of osteogenesis, and causes the acceleration of the nucleation kinetics of HA and inhibits its crystallization process. In 2006, Landi et al. observed that Mg-substituted hydroxyapatite improved the behavior of cells in terms of adhesion, proliferation, and metabolic activity, as compared to HA. 
Zinc is a major trace element in bone, and has been found to play a major role in human tissue development. Zinc-substituted HA is a potential material where zinc inhibits bone resorption and has a stimulatory effect on bone formation. When zinc was substituted into the HA and TCP crystal lattices, it was found to inhibit osteoclasts and to promote bone growth.
Despite the beneficial results, the clinical applications of ion-substituted ceramics and cements are limited due to their low mechanical strength. By coating implants with ion-substituted HA, the higher mechanical strength of the metal can be combined with the properties of the ion-substituted HA. Therefore, the beneficial biological effects of the bioactive coatings makes them suitable to be applied with biomedical implants. 
| Conclusions|| |
Overall, specific biomaterials/biomimetics were found to be more effective than OFD in improving the attachment levels in intraosseous defects. Difference in CAL gain varied greatly with respect to different biomaterials/biomimetic agents. Due to a significant heterogeneity in results between studies in most treatment groups, general conclusions about the expected clinical benefit of graft biomaterials need to be interpreted with caution. Biomaterial-supplemented reconstructive procedures are associated with positive treatment as compared to OFD, but ceramics are used as void fillers or scaffolds and cannot be used in areas of high stress or function unless they are combined with an osteogenic or osteoinductive bone graft material.
The biological effects of the bioactive coatings makes them suitable to be applied with biomedical implants; fluoride and HA coatings are being used since a decade as implant coatings and research is ongoing for using other ions in ion-substituted ceramics. Research on stem cells and synthetic bone graft materials pertaining to regeneration is still in its infancy. A lot of research has been done on calcium phosphate ceramics and their action on stimulating bone re-growth by attracting stem cells and growth factors to promote healing and integration of grafted tissue. Soon scientists will be developing a material for bone grafts that could one day replace the "gold standard" natural bone implants. 
| References|| |
Stevens MM. Biomaterials for bone tissue engineering. Mater Today 2008;11:18-25.
Benyus JM, Morrow W. Biomimicry: Innovation Inspired by Nature. Perennial, New York: William Morrow Paperbacks; 2002. p. 215.
Biomimetics. Available from: http://www.inotektextiles.com/technology.[Last accessed on 2013 Mar 19].
Nano-biomimetics. Available from: http://www.stanford.edu/group/mota/education/Physics%2087N%20Final%20Projects/Group%20Gamma/index.htm. [Last accessed on 2013 Mar 18].
Biomimetics - NCRM- NICHI - In Classification. Available from: http://www.ncrm.org/biomimetics.htm. [Last accessed on 2013 Mar 18].
Hannouche D, Petite H, Sedel L. Current trends in the enhancement of fracture healing. J Bone Joint Surg Br 2001;83:157-64.
Brydone AS, Meek D, Maclaine S. Bone grafting, orthopaedic biomaterials, and the clinical need for bone engineering. Proc Inst Mech Eng H 2010;224:1329-43.
Parikh SN. Bone graft substitutes: Past, present, future. J Postgrad Med 2002;48:142-8.
Gentleman E, Ball MD, Stevens MM. Biomaterials. Medical Sciences. Vol. 2; p. 3. Encyclopedia of life support systems. Available from: http://www.eolss.net/Sample-Chapters/C03/E6-59-13-07.pdf [Last accessed on 2013 Mar 22].
Darby I. Periodontal materials. Australian Dental Journal 2011;56 Suppl 1:107-18.
Bueno EM, Glowacki J. Cell-free and cell-based approaches for bone regeneration. Nat Rev Rheumatol 2009;5:685-97.
Heness G, Ben-Nissan B. Innovative bioceramics. Mater Forum 2004;27:104-14.
Paolantonio M, Perinetti G, Dolci M, Perfetti M, Tetè S, Sammartino G, et al. Surgical treatment of periodontal intrabony defects with calcium sulfate implant and barrier versus collagen barrier or open flap debridement alone: A 12-month randomized controlled clinical trial. J Periodontol 2008;79:1886-93.
Orsini M, Orsini G, Benlloch D, Aranda JJ, Sanz M. Long-term clinical results on the use of bone-replacement grafts in the treatment of intrabony periodontal defects. Comparison of the use of autogenous bone graft plus calcium sulfate to autogenous bone graft covered with a bioabsorbable membrane. J Periodontol 2008;79:1630-7.
Yamamiya K, Okuda K, Kawase T, Hata K, Wolff F, Yoshie H. Tissue-engineered cultured periosteum used with platelet-rich plasma and hydroxyapatite in treating human osseous defects. J Periodontol 2008;79:811-8.
Kasaj A, Röhrig B, Zafiropoulos GG, Willershausen B. Clinical evaluation of nanocrystalline hydroxyapatite paste in the treatment of human periodontal bony defects--a randomized controlled clinical trial: 6-month results. J Periodontol 2008;79:394-400.
Gupta J, Gil AS, Sikri P. Evaluation of the relative efficacy of an alloplast used alone and in conjunction with an osteoclast inhibitor in the treatment of human periodontal infrabony defects: A clinical and radiological study. Indian J Dent Res 2011;22:225-31.
Menezes LM, Rao J. Long-term clinical evaluation of platelet-rich plasma in the treatment of human periodontal intraosseous defects: A comparative clinical trial. Quintessence Int 2012;43:571-82.
Stein JM, Fickl S, Yekta SS, Hoischen U, Ocklenburg C, Smeets R. Clinical evaluation of a biphasic calcium composite grafting material in the treatment of human periodontal intrabony defects: A 12-month randomized controlled clinical trial. J Periodontol 2009;80:1774-82.
Meyle J, Hoffman T, Topoll H, Jervøe-Storm PM, et al
. A multi-centre randomized controlled clinical trial on the treatment of intra-bony defects with enamel matrix derivatives/synthetic bone graft or enamel matrix derivatives alone: Results after 12 months. J Clin Periodontol 2011;38:652-60.
Pietruska M, Pietruski J, Nagy K, Brecx M, Arweiler NB, Sculean A. Four-year results following treatment of intrabony periodontal defects with an enamel matrix derivative alone or combined with a biphasic calcium phosphate. Clin Oral Investig 2012;16:1191-7.
Thakare K, Deo V. Randomized controlled clinical study of rhPDGF-BB + β-TCP versus HA + β-TCP for the treatment of infrabony periodontal defect: Clinical and Radiographic results. Int J Periodontics Restorative Dent 2012;32:689-96.
Lee MJ, Kim, BO, Yuj SJ. Clinical evaluation of a biphasic calcium phosphate grafting material in the treatment of human periodontal intrabony defects. J Periodontal Implant Sci 2012;42:127-35.
Dori F, Arweiler NB, Szántó E, Agics A, Gera I, Sculean A. Ten-year results following treatment of intrabony defects with an enamel matrix protein derivative combined with either a natural bone mineral or a β-tricalcium phosphate. J Periodontol 2013;84:749-57.
Nevins M, Giannobile WV, McGuire MK, Kao RT, Mellonig JT, Hinrichs JE, et al
. Platelet-derived growth factor stimulates bone fill and rate of attachment level gain: Results of a large multicenter randomized trial. J Periodontal 2005;76:2205-15.
Saini N, Sikri P, Gupta H. Evaluation of the relative efficacy of autologous platelet-rich plasma in combination with β-tricalcium phosphate alloplast versus an alloplast alone in the treatment of human periodontal infrabony defects: A clinical and radiological study. Indian J Dent Res 2011;22:107-15.
Jayakumar A, Rajababu P, Rohini S, Butchibabu K, Naveen A, Reddy PK, et al
. Multi-centre, randomized clinical trial on the efficacy and safety of recombinant human platelet-derived growth factor with β-tricalcium phosphate in human intra-osseous periodontal defects. J Clin Periodontol 2011;38:163-72.
Windisch P, Stavropoulos A, Molnár B, Szendröi-Kiss D, Szilágyi E, Rosta P, et al
. A phase IIa randomized controlled pilot study evaluating the safety and clinical outcomes following the use of rhGDF-5/β-TCP in regenerative periodontal therapy. Clin Oral Investig 2012;16:1181-9.
Nevins M, Kao RT, McGuire MK, McClain PK, Hinrichs JE, McAllister BS, et al
. Platelet-derived growth factor promotes periodontal regeneration in localized osseous defects: 36-month extension results from a randomized, controlled, double-masked clinical trial. J Periodontol 2013;84:456-64.
De Leonardis D, Paolantonio M. Enamel matrix derivative, alone or associated with a synthetic bone substitute, in the treatment of 1- to 2-wall periodontal defects. J Periodontol 2013;84:444-55.
Shirakata Y, Setoguchi T, Machigashira M, Matsuyama T, Furuichi Y, Hasegawa K, et al
. Comparison of injectable calcium phosphate bone cement grafting and open flap debridement in periodontal intrabony defects: A randomized clinical trial. J Periodontol 2008;79:25-32.
Rajesh JB, Nandakumar K, Varma HK, Komath M. Calcium phosphate cement as a "barrier-graft" for the treatment of human periodontal intraosseous defects. Indian J Dent Res 2009;20:471-9.
Park JS, Suh JJ, Choi SH, Moonn IS, Cho KS, Kim CK, et al
. Effects of pretreatment clinical parameters on bioactive glass implantation in intrabony periodontal defects. J Periodontol 2001;72:730-40.
Mengel R, Schreiber D, Flores-de-Jacoby L. Bioabsorbable membrane and bioactive glass in the treatment of intrabony defects in patients with generalized aggressive periodontitis: Results of a 5-year clinical and radiological study. J Periodontol 2006;77:1781-7.
Demir B, Sengün D, Berberoğlu A. Clinical evaluation of platelet-rich plasma and bioactive glass in the treatment of intra-bony defects. J Clin Periodontol 2007;34:709-15.
Leknes NK, Andersen KM, Bøe OE, Skavland RJ, Albandar JM. Enamel matrix derivative versus bioactive ceramic filler in the treatment of intrabony defects: 12-month results. J Periodontol 2009;80:219-27.
Yadav VS, Narula SC, Sharma RK, Tewari S, Yadav R. Clinical evaluation of guided tissue regeneration combined with autogenous bone or autogenous bone mixed with bioactive glass in intrabony defects. J Oral Sci 2011;53:481-8.
Kumar PG, Kumar JA, Anumala N, Reddy KP, Avula H, Hussain SN. Volumetric analysis of intrabony defects in aggressive periodontitis patients following use of a novel composite alloplast: A pilot study. Quintessence Int 2011;42:375-84.
Armitage GC. Clinical evaluation of periodontal diseases. Periodontol 2000; 1995:7:39-53.
Borden M, Attawia M, Khan Y, Laurencin CT. Tissue engineered microsphere-based matrices for bone repair: Design and evaluation. Biomaterials 2002;23:551-9.
Bohner M. Resorbable biomaterials as bone graft substitutes. Mater Today 2010;13;24-30.
Pecora G, Andreana S, Margarone JE 3 rd
, Covani U, Sottosanti JS. Bone regeneration with a calcium sulfate barrier. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1997;84:424-9.
Strocchi R, Orsini G, Iezzi G, Scarano A, Rubini C, Pecora G, et al
. Bone regeneration with calcium sulfate: Evidence for increased angiogenesis in rabbits. J Oral Implantol 2002;28:273-8.
Budhiraja S, Bhavsar N, Kumar S, Desai K, Duseja S. Evaluation of calcium sulphate barrier to collagen membrane in intrabony defects. J Periodontal Implant Sci 2012;42:237-42.
Hu J, Fraser R, Russell JJ, Ben-Nissan B, Vago R. Australian coral as a biomaterial: Characteristics. J Mater Sci Technol 2000;16:591-5.
46 Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL. Regeneration of periodontal tissue: Bone replacement grafts. Dent Clin North Am 2010;54:55-71.
Moore WR, Graves SE, Bain GI. Synthetic bone graft substitutes. ANZ J Surg 2001;71:354-61.
Bartee BK. Implant Site Development and Extraction Site Grafting Bone Biology and Physiology, Selection of Grafting Materials, Selection of Barrier Membranes and Surgical. [Available from: http://www.deorematerials.com/wordpress/wp-content/uploads/2011/11/implant-site-development.pdf. [Last accessed on 2014 May 30].
Tonino AJ, van der Wal BC, Heyligers IC, Grimm B. Bone remodeling and hydroxyapatite resorption in coated primary hip prostheses. Clin Orthop Relat Res 2009;467:478-84.
Checchi V, Savarino L, Montevecchi M, Felice P, Checchi L. Clinical-radiographic and histological evaluation of two hydroxyapatites in human extraction sockets: A pilot study. Int J Oral Maxillofac Surg 2011;40:526-32.
Horvath A, Stavropoulos A, Windisch P, Luckas L, Gera I, Sculean A. Histological evaluation of human intrabony periodontal defects treated with an unsintered nanocrystalline hydroxyapatite paste. Clin Oral Investig 2013;17:423-30.
Stavropoulos A, Windisch P, Szendröi-Kiss D, Peter R, Gera I, Sculean A. Clinical and histologic evaluation of granular Beta-tricalcium phosphate for the treatment of human intrabony periodontal defects: A report on five cases. J Periodontol 2010;81:325-34.
Ridgway HK, Mellonig JT, Cochran DL. Human histologic and clinical evaluation of recombinant human platelet-derived growth factor and beta-tricalcium phosphate for the treatment of periodontal intraosseous defects. Int J Periodontics Restorative Dent 2008;28:171-9.
Torres J, Tamimi F, Alkhraisat M, Prados-Frutos JC, Lopez-Cabarcos E. Bone substitutes. In: Turkyilmaz I, editor. Implant Dentistry - The Most Promising Discipline of Dentistry. CBS Publishers; 2011. Available from: http://www.intechopen.com/books/implant-dentistry-the-most-promising-discipline-of-dentistry/bone-substitutes [Last assessed at 2013 Jun 01].
Komath M, Varma HK. Current status of dental materials research in SCTIMST, Trivandrum Part II: Synthetic bone graft materials. Kerala Dental J 2009;32:193-8.
Sarkar MR, Wachter N, Patka P, Kinzl L. First histological observations on the incorporation of a novel calcium phosphate bone substitute material in human cancellous bone. J Biomed Mater Res 2001;58:329-34.
Hench LL, Jones JR, Sepulveda P. Bioactive materials for tissue engineering scaffolds. In: Polok JM, Hench LL, Kemp P, editors. Future Strategies for Tissue and Organ Replacement. London: Imperial College Press; 2002. p. 3-24.
Umesh Y. Comparison of Demineralized Freeze Dried Bone Allograft (Dynagraft) with Bioactive Glass (Perioglas) for Treating Periodontal Osseous Defects Humans: A Clinical and Radiographic Study. Karnataka, India: Rajiv Gandhi University 0f Health Sciences; 2003-6. p. 15.
Greenspan DC. Bioactive glass: mechanisms of bone bonding Dental appointment 1999; 91: 8.
Nevins ML, Camelo H, Mevins H, et al
. Human histologic evaluation of bioactive ceramics in the treatment of periodontal osseous defects. Int J Periodont Rest Dent. 2000; 20: 459-467.
Biomatcell AB, Xia W, Lindahl C, Engqvist H, Thomsen P, Lausmaa J. Ion Substituted Calcium Phosphate Coatings. WO 2010126436 A1. Patent No: WIPO Patent Application WO/2010/126436. 2010.
Najda J, Gmiński J, Drózdz M, Danch A. The action of excessive, inorganic silicon (Si) on the mineral metabolism of calcium (Ca) and Magnesium (Mg). Biol Trace Elem Res 1993;37:107-14.
Pietak AM, Reid JW, Stott MJ, Sayer M. Silicon substitution in the calcium phosphate bioceramics. Biomaterials 2007;28:4023-32.
Waked W, Grauer J. Silicates and bone fusion. Orthopedics 2008;31:591-7.
Gross KA, Rodríguez-Lorenzo LM. Sintered hydroxylfluorapatites. Part I: Sintering ability of precipitated solid solution powders. Biomaterials 2004;25:1375-84.
Robinson C, Shore RC, Brookes SJ, Strafford S, Wood SR, Kirkham J. The chemistry of enamel caries. Crit Rev Oral Biol Med 2000;11:481-95.
Landi E, Tampieri A, Celotti G, Sprio S, Sandri M, Logroscino G. Sr-substituted hydroxyapatites for osteoporotic bone replacement. Acta Biomater 2007;3:961-9.
Landi E, Tampieri A, Mattioli-Belmonte M, Celotti G, Sandri M, Gigante A, et al
. Biomimetic Mg- and Mg,CO3-substituted hydroxyapatites: Synthesis charcterization and in vitro behaviour. J Euro Ceram Soc 2006;26:2593-601.
Yuan H, Fernandes H, Habibovic P, de Boer J, Barradas AM, de Ruiter A, et al
. Osteoinductive ceramics as a synthetic alternative to autologous bone grafting. Proc Natl Acad Sci U S A 2010;107:13614-9.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2]
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