|Year : 2017 | Volume
| Issue : 2 | Page : 90-94
|Assessment of effectiveness of fluconazole and clotrimazole in treating oral candidiasis patients: A comparative study
RC Jagat Reddy1, S Jeelani2, P Duraiselvi2, M Kandasamy3, G Suresh Kumar4, R Azhal Vel Pandian2
1 Department of Oral Medicine and Radiology, Chettinad Dental College and Research Institute, Kelambakkam, Tamil Nadu, India
2 Department of Oral Medicine and Radiology, Sri Venkateshwaraa Dental College, Puducherry, India
3 Department of Oral Medicine and Radiology, Rajas Dental College and Hospital, Tirunelveli, Tamil Nadu, India
4 Department of Oral and Maxillofacial Surgery, Adthiparasakthi Dental College and Hospital, Melmaruvathur, Tamil Nadu, India
|Date of Submission||24-Jan-2017|
|Date of Acceptance||28-Feb-2017|
|Date of Web Publication||29-Mar-2017|
R C Jagat Reddy
Department of Oral Medicine and Radiology, Chettinad Dental College and Research Institute, Kelambakkam, Tamil Nadu
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Aims: One of the most common fungal infections infecting humans is Candidiasis. Belonging to the group of opportunistic infections, it often affects individuals with various debilitating diseases. Fluconazole and clotrimazole are two of the commonly used anti-fungal agents for the treatment of oral candidiasis. Hence, we planned this study to evaluate the effectiveness of fluconazole and clotrimazole in the treatment of patients suffering from candidiasis.
Materials and Methods: A total of 180 participants were enrolled in the present study. All the patients of candidiasis were divided broadly into two study groups. Group I included patients who were treated with fluconazole mouthrinse whereas group II included patients who were treated with clotrimazole mouth paint. Grading of patient discomfort was done as noted from readings given by the patients. Specimen was collection by a swab from the lesional area of the oral cavity from the patients and were incubated in Sabouraud's dextrose agar medium and assessed. All the patients were treated with medication as give to their respective groups. Patients were recalled as assessed. All the readings were recorded and analyzed.
Results: For group I patients, the fungal eradication was 89.5%, whereas for group II patients, the fungal eradication was 86.7%. No significant results were obtained while comparing the mycological eradiation in patients of the two study groups.
Conclusion: Approximately similar effectiveness in terms of treatment was noted with fluconazole and clotrimazole in treating patients with candidiasis.
Keywords: Candidiasis, clotrimazole, fluconazole
|How to cite this article:|
Jagat Reddy R C, Jeelani S, Duraiselvi P, Kandasamy M, Kumar G S, Pandian R A. Assessment of effectiveness of fluconazole and clotrimazole in treating oral candidiasis patients: A comparative study. J Int Soc Prevent Communit Dent 2017;7:90-4
|How to cite this URL:|
Jagat Reddy R C, Jeelani S, Duraiselvi P, Kandasamy M, Kumar G S, Pandian R A. Assessment of effectiveness of fluconazole and clotrimazole in treating oral candidiasis patients: A comparative study. J Int Soc Prevent Communit Dent [serial online] 2017 [cited 2018 Jan 20];7:90-4. Available from: http://www.jispcd.org/text.asp?2017/7/2/90/203321
| Introduction|| |
Candida genus, in particular Candida albicans, is the one of the common cause of yeast-associated oral infections.  There occurrence has been widely described in the literature. Over the last four to five decades, the frequency and incidence of occurrence of candidiasis has significantly increased. The main reason that might attribute for such an increase in its incidence might be the increase in the prevalence of human immunodeficiency virus (HIV) and other immuno-compromising states and diseases. It also occurs more frequently among elderly individuals. 
More than 150 different species of asporogenous "yeast-like" fungi comprises Candida genus. The distribution of all the members of this genus is ubiquitous and consists of inhabitation of soil as saprophytes, aquatic environment, and even colonization of various animal reservoirs. At 37°C, the growth of most of Candida spices is prohibited, and therefore, human colonization is not associated with it under normal conditions. 
Within humans, several species persist as commensal microorganism and can act as potential opportunistic pathogen in compromised body status. With respect to the treatment of these oral fungal infections, the most common line of treatment is the removal of the etiologic factor along with suitable anti-fungal therapy. 
Few of the routinely employed anti-fungal agents include fluconazole and clotrimazole. There is a paucity of data regarding the comparative evaluation of the abovementioned anti-fungal agents in the treatment of oral candidiasis.  Hence, we planned this study to evaluate the effectiveness of fluconazole and clotrimazole in the treatment of patients suffering from candidiasis.
| Materials and Methods|| |
The present study was conducted in the department of oral medicine and radiology of the dental institute and included assessment of 180 participants with chief problem of oral candidiasis from 2010 to 2015. Ethical approval was obtained from the institutional ethical committee and written consent was obtained after explaining in detail the entire research protocol. Complete physical examination of the patients was done before starting the treatment therapy of the patients. Recording of the time duration of the candidiasis was done along with other personal details of the patients. Exclusion criteria for the present study included:
- Pregnant patients,
- Patients who underwent any kind of anti-fungal therapy in the past 1 month,
- Patients who were on barbiturates or anticoagulants in the past 1 month,
- Patients with any known drug allergy,
- Patients with alcohol history,
- Patients with history of any psychiatric disorder.
All the patients were broadly divided into two study groups. Group I included patients who were under treatment with fluconazole therapy whereas group II included patients who were on clotrimazole therapy. Based on the clinical history, visual examination of the lesion area and presence of clinical signs and symptoms, diagnosis of the candidiasis was made. Both the signs and symptoms of candidiasis were graded by patients into the following categories based on the severity of the lesion and individual perception:
- Moderate, and
Symptoms' severity was categorized on the basis patient's response to discomfort whereas sign's severity was categorized on the basis of extent of lesion. Mild referred to cases which involved localized involvement to one or two oral sites, moderate referred to localized involvement of more than two oral sites, whereas severe cases involved generalized oral candidiasis. Microbiological culture assessment was done to confirm the diagnosis of oral candidiasis. Swab was taken from the lesion area of the patients and was transferred to the microbiological laboratory in the transport medium for culturing. They were incubated in Sabouraud's dextrose agar medium for assessment of culture growth characteristics. All the samples were incubated in the culture medium at 37°C for 1-2 days. Counting of the yeast colonies was done 48 hours after incubation.
In group I patients, preparation of fluconazole suspension was done and were given to all the patients in the form of prepared moth rinse. Patients were instructed to use the suspension mouth rinse three times a day. In group II patient, suspension of clotrimazole was given in the form of moth paint and patients were instructed to use it thrice daily. Recalling of the patients was done after 2 weeks of continuation of the treatment therapy and was examined thoroughly for the presence of clinical signs and symptoms; microbial growth was assessed by culturing swab specimens as done earlier before the starting of the treatment. All the results were analyzed by PASW Statistics for Windows, Version 18.0. Chicago: SPSS Inc. Chi-square test and student's t-test was used for the assessment of level of significance. P-value of less than 0.05 was taken as significant. All samples were examined twice to avoid intraobserver variability and kappa value came out to be 0.8.
| Results|| |
In both the study groups, prolonged antibiotic therapy was the most common compromised state associated with the presence of fungal infections [Graph 1 [Additional file 1] ]. Mean age of the patients in groups I and II was 49.5 years and 51.2 years, respectively [Table 1] and [Graph 2 [Additional file 2] ]. In group I, 65% of the patient population was of males whereas remaining 35% were females. Among group II, 62% of patient population was of males while the reaming was of females. Among group I patients, before starting the treatment, 81% of the patient population had moderate severity of clinical symptoms, whereas in group II, 75% of the patient population had moderate severity of symptoms [Table 2] and [Graph 3 [Additional file 3] ]. Among group I participants, after commencing the treatment, in 98% of the patients, clinical signs and symptoms were absent whereas in group II participants, 88% of the patients showed absence of clinician signs. Significant results were obtained while comparing the signs and symptoms of the patients in the two study groups after the treatment. In group I patients, before and after the treatment, the colony count was 1325.25 and 9.51, respectively, whereas in the group II patients, the mean colony count was 996.52 and 21.82, respectively [Table 3]. Significant results were obtained while comparing the mean colony count before and after the treatment in both study groups.
|Table 1: Comparative assessment of the two study groups in terms of various parameters|
Click here to view
|Table 2: Comparison of various clinical signs and symptoms in between the patients of the two study group|
Click here to view
|Table 3: Comparison of colony counts before and after the treatment therapy|
Click here to view
| Discussion|| |
Over the past few decades, there has been an increase in the incidence of occurrence of fungal infections.  Furthermore, there has been as simultaneous increase in the incidence and occurrence of the predisposing factors.  Some of the important predisposing factors included xerostomia, antibiotic therapy for prolonged period of time, local chronic trauma or irritation, endocrinal disorders, medically compromised states, etc.  One of the common fungal infection included oral candidiasis. Treatment of the fungal infections included removal of the etiologic systemic or local factor along with antifungal therapy. Fluconazole and clotrimazole are the two commonly used anti-fungal agents for the treatment of fungal lesions. ,, Hence, we aimed to evaluate the effectiveness of fluconazole and clotrimazole in the treatment of patients suffering from candidiasis.
In the present study, we observed significant results while comparing the mean difference of colony culture growth before and after the treatment in both the study groups. Similar results were obtained by Sholapurkar et al. who observed similar findings in their study.  O-Prasertsawat et al. comparatively evaluated the effectiveness of fluconazole and clotrimazole in the treatment of vulvovaginal candidiasis. They assessed 103 female patients in a single blinded randomized trial and divided them broadly into two study groups. First group consisted of 53 patients and included participants in whom treatment was done by fluconazole, while the other group consisted of 50 patients and included those in which treatment was done by clotrimazole. They did not observe any significant difference in relation to the clinical characteristic in between the two study groups. They observed approximately 79% and 80% mycological cure rates in the two study groups, respectively. They concluded that for the treatment of cases of vulvovaginal candidiasis, fluconazole can be given as an alternative line of treatment. 
Sekhavat et al. comparatively evaluated the effectiveness of single dose of fluconazole and intravaginal clotrimazole 200 mg per day for 6 days in the patients undergoing treatment for the acute episode of vulvovaginal candidiasis (VVC). They prospectively analyzed 142 patients with were diagnosed with VVC and divided them randomly into two study groups. First group consisted of 70 patients and included those patients who received intravaginal tablet whereas the other group included 72 patients and consisted of participants who were given single dose oral fluconazole. They observed that, at the time of follow-up, during the second visit of the patients, approximately 85% and 81% of the patients of the group receiving fluconazole were cured clinically and mycologically, respectively. Similarly in the other study group, approximately 83% and 70% of the study group patients were cured clinically and mycologically, respectively. From the results, they concluded that for the treatment of cases of VVC, oral fluconazole single dose appeared to be a valid mode of treatment. 
Goins et al. compared the effectiveness of nystatin and fluconazole in treating the cases of oral candidiasis. One of the common conditions affecting the young infant group of individuals is oral thrush. One of the frequent problems associated with the administration of nystatin is the frequent cases of recurrence and difficulty encountered in its administration. They randomly analyzed 34 infants and were randomly subjected to either nystatin oral suspension four times a day for 10 days or fluconazole suspension 3 mg/kg in a single daily dose for 7 days. They observed that among the patients treated with nystatin, the clinical curing rate was 32%, while in the other group, a success rate of 100% was observed. Significant results were obtained while comparing the two anti-fungal agents. From the results, they concluded that superior action of treatment is associated with fluconazole.  Sholapurkar et al. assessed the efficacy of fluconazole mouthrinse and clotrimazole mouthpaint in the treatment of cases of oral candidiasis. They observed significant difference while comparing the fungal growth on culture media before and after the treatment. 
Strength of the study
Authors advocate the use of fluconazole and clotrimazole for treating oral candidiasis patients, thereby helping in improving the quality of life of the patients.
Limitations of the study
- Sample size was small
- Underlying etiologic factors for the occurrence of candidiasis were not explored.
The treatment therapy might differ depending upon the etiologic cause of candidiasis which was not taken as a parameter for the present study.
| Conclusion|| |
From the above results, the authors obtained significant results while using both the fluconazole and clotrimazole in treating patients with oral candidiasis. However, future studies are required in the same field for better exploration of results.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Lyu X, Zhao C, Yan Z, Hua H. Efficacy of nystatin for the treatment of oral candidiasis: A systematic review and meta-analysis. Drug Des Devel Ther 2016;10:1161-71.
Carmello JC, Alves F, G. Basso F, de Souza Costa CA, Bagnato VS, Mima EG, et al
. Treatment of Oral Candidiasis Using Photodithazine ®
- Mediated Photodynamic Therapy In Vivo
. PLoS One 2016;11:e0156947.
Odds FC. Morphogenesis in Candida albicans. Critical reviews in microbiology 1985;12:45-93.
Kumar R, Saraswat D, Tati S, Edgerton M. Novel Aggregation Properties of Candida albicans Secreted Aspartyl Proteinase Sap6 Mediate Virulence in Oral Candidiasis. Infect Immun 2015;83:2614-26.
Velliyagounder K, Alsaedi W, Alabdulmohsen W, Markowitz K, Fine DH. Oral lactoferrin protects against experimental candidiasis in mice. J Appl Microbiol 2015;118:212-21.
Armstrong AW, Bukhalo M, Blauvelt A. A Clinician's Guide to the Diagnosis and Treatment of Candidiasis in Patients with Psoriasis. Am J Clin Dermatol 2016;17:329-36.
Patil S, Rao RS, Majumdar B, Anil S. Clinical Appearance of Oral Candida Infection and Therapeutic Strategies. Front Microbiol 2015;6:1391.
Conti HR, Whibley N, Coleman BM, Garg AV, Jaycox JR, Gaffen SL. Signaling through IL-17C/IL-17RE Is Dispensable for Immunity to Systemic, Oral and Cutaneous Candidiasis. PLoS One 2015;10:e0122807.
Yan Z, Liu X, Liu Y, Han Y, Lin M, Wang W, et al
. The Efficacy and Safety of Miconazole Nitrate Mucoadhesive Tablets versus Itraconazole Capsules in the Treatment of Oral Candidiasis: An Open-Label, Randomized, Multicenter Trial. PLoS One 2016;11:e0167880.
Kong EF, Tsui C, Boyce H, Ibrahim A, Hoag SW, Karlsson AJ, et al
. Development and In Vivo Evaluation of a Novel Histatin-5 Bioadhesive Hydrogel Formulation against Oral Candidiasis. Antimicrob Agents Chemother 2016;60:881-9.
Williams D, Lewis M. Pathogenesis and treatment of oral candidosis. J Oral Microbiol 2011;28:3.
Sholapurkar AA, Pai KM, Rao S. Comparison of efficacy of fluconazole mouthrinse and clotrimazole mouthpaint in the treatment of oral candidiasis. Aust Dent J 2009;54:341-6.
O-Prasertsawat P, Bourlert A. Comparative study of fluconazole and clotrimazole for the treatment of vulvovaginal candidiasis. Sex Transm Dis 1995;22:228-30.
Sekhavat, Leila et al
. Oral fluconazole 150 mg single dose versus intra-vaginal clotrimazole treatment of acute vulvovaginal candidiasis. J Infect Pub Health 2011;4:195-9.
Goins RA, Ascher D, Waecker N, Arnold J, Moorefield E. Comparison of fluconazole and nystatin oral suspensions for treatment of oral candidiasis in infants. Pediatr Infect Dis J 2002;21:1165-7.
[Table 1], [Table 2], [Table 3]
| Article Access Statistics|
| Viewed||663 |
| Printed||18 |
| Emailed||0 |
| PDF Downloaded||46 |
| Comments ||[Add] |