|Year : 2017 | Volume
| Issue : 6 | Page : 370-376
|Effect of popping chocolate and candy on enamel microhardness of primary and permanent teeth
Mitra Tabari1, Homayoon Alaghemand2, Durdi Qujeq3, Elahe Mohammadi4
1 Dental Materials Research Center, Institute of Health; Assistant Professor of Pediatric Dentistry, Babol University of Medical Sciences, Babol, Iran
2 Dental Materials Research Center, Institute of Health; Assistant Professor of Operative and Esthetic Dentistry, Babol University of Medical Sciences, Babol, Iran
3 Cellular and Molecular Biology Research Center, Health Research Institute; Assistant Professor of Clinical Biochemistry, Babol University of Medical Sciences, Babol, Iran
4 Student Research Committee, Babol University of Medical Sciences, Babol, Iran
|Date of Submission||25-Oct-2017|
|Date of Acceptance||02-Dec-2017|
|Date of Web Publication||29-Dec-2017|
Dr. Elahe Mohammadi
Babol University of Medical Sciences, Ganj Afrooz Ave, Babol
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Aims and Objectives: Dental erosion is a common disease in children. Food diets, due to high amounts of juice, soft drinks, chewing gum, and acidic chocolate, are one of the most important risk factors in erosive processes among children. The aim of this study was to evaluate the effect of candy and chocolate on the microhardness of tooth enamel.
Materials and Methods: Two types of popping candy and one type of popping chocolate were used in this study. Thirty-three healthy permanent premolar teeth and 33 primary incisor teeth (A or B) were selected. Five grams of each popping chocolate or candy was dissolved with 2 ml of artificial saliva. Subsequently, their pH and titrable acidity (TA) as well as microhardness and surface roughness of enamel were examined in the laboratory. Data were analyzed and evaluated Released 2011. IBM SPSS Statistics for Windows, Version 20.0. Armonk, NY through independent t-test, paired t-test, Tukey test, and ANOVA.
Results: The results of this study showed that only the pH of the candies was below the critical pH of the enamel (5.5) and their TA was B = 0.20 and C = 0.21. The most significant effect on the enamel microhardness of the permanent and primary teeth was by the following types of candy: orange flavor (C), strawberry flavor (B), and chocolate (A), respectively. This difference was significant (P < 0.001) and the surface roughness increased after exposure.
Conclusions: This study showed that popping chocolate and candy reduces microhardness of enamel.
Keywords: Candy, chocolate, enamel, hardness
|How to cite this article:|
Tabari M, Alaghemand H, Qujeq D, Mohammadi E. Effect of popping chocolate and candy on enamel microhardness of primary and permanent teeth. J Int Soc Prevent Communit Dent 2017;7:370-6
|How to cite this URL:|
Tabari M, Alaghemand H, Qujeq D, Mohammadi E. Effect of popping chocolate and candy on enamel microhardness of primary and permanent teeth. J Int Soc Prevent Communit Dent [serial online] 2017 [cited 2020 Apr 7];7:370-6. Available from: http://www.jispcd.org/text.asp?2017/7/6/370/222075
| Introduction|| |
After eruption, three types of acid dissolution may remove tooth minerals: decay, erosion, and acid etching used for retention of resin fill materials. Loss of dental structure might occur in a variety of forms, including abrasion, attrition, abfraction, demastication, fine cracks, and resorption. Dental erosion is a chemical process where the hard surface is dissolved by acidic processes, and the microorganisms do not interfere with it.
Dental erosion is a common disease in children. Early erosive damage to teeth may cause severe tooth surface loss, tooth sensitivity, over closure, and poor esthetics. Therefore, early diagnosis of the disease and appropriate preventive measures are important. Awareness of the etiological factors of dental erosion is a prerequisite for such actions. In children and adolescents (such as adults), external and internal factors or a combination of them is possible reason for this disease. When hard dental tissue is exposed to an unsaturated solution, both hydroxyapatite and fluorapatite are dissolved and the teeth become affected by erosion. At pH below the critical pH of the enamel (pH = 5.5), tooth minerals tend to demineralization while they tend to be remineralized at pH above the critical pH level.
Wang et al. with evaluation of erosive potential of soft drinks on human enamel reported that the pH values of the soft drinks were below the critical pH value and low pH value causes more surface enamel loss.
Etiological factors of dental erosion are divided into intrinsic and extrinsic groups: intrinsic factors due to gastric acid that enters the oral cavity through the gastric reflux or vomiting and extrinsic factors due to environmental causes, diet, medications, and lifestyle. The current diet, due to high consumption of juice, acid beverages, some acidic center-filled chewing gums, and acidic candies, is the most important risk factor for erosive processes among children.,, The final erosive potential of food and drink depends on the contrast between the chemical properties (pH, total acidity, calcium and the amount of phosphate, and adhesion), biological factors (salivary flow rate, buffering capacity and composition, pellicle formation, tooth composition, and soft-tissue anatomy), and behavioral factors (eating and drinking habits, especially continuous and prolonged ones). Among these factors, pH, titration acidity, and calcium concentrations are the most important in determining the potential of erosive materials. Popping candy is producted from a mixture of sucrose, lactose, and corn starch dissolved in water, and then, the solution is melted to the point, it includes 2%–3% water, and then, carbon dioxide (CO2) is exposed to molten sugar at high pressure (625–675 psi), followed by the mixture being cooled. When the candy is kept inside the mouth, it melts and gas scapes which creates short feelings of entertainment and amusement.
Mudumba et al. 2014 evaluated and compared the change in the microhardness of the enamel after exposure to the acidic center-filled chewing gums in permanent and primary teeth. It was concluded that in all groups exposed to chewing gum, a clear decrease was seen in the microhardness and both types of chewing gum equally erosive. Davies et al. evaluated the erosive potential of a number of commercially available sour sweets in vitro. In this study, the erosive potential was examined by measuring pH and neutralizable acidity as well as the ability of erosion in permanent and primary enamel (using profilometry), and then, they compared the parameters with orange juice as the control group. It was concluded that all sour candies are erosive even some are more erosive than orange juice. Since there has not been any research on popping chocolates in the oral environment and its effect on permanent and primary teeth, this study evaluates the effect of these chocolates on the microhardness of enamel.
| Materials and Methods|| |
The present study was carried out in the Dental Material Research Center, Babol University of Medical Science, Babol, Iran, in 2016, and approved by the Ethics Committee of Babol University of Medical Science, Babol, Iran (MUBABOL. REC.1396.11). In this experimental study, two types of popping candy and one type of popping chocolate were used that are commonly available to the public. Popping chocolate and candy compounds are shown in [Table 1] according to the factory report.
|Table 1: Types, compounds, and the manufacturing country of the materials|
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Before the experiment, each chocolate or candy was crushed with a pestle, and 5 g of each popping chocolate/candy was dissolved in 2 ml of artificial saliva with 40°C., Then, their pH and titrable acidity (TA) as well as microhardness and surface roughness of enamel were examined in the laboratory.
The pH of the solutions was measured at room temperature by a digital pH meter (Basic 20+, Crison Instruments, SA, Barcelona, Spain). First, the pH meter was calibrated with a measurement error of 0.01 using standard buffers at pH 9.25, pH 7.02, and pH 4. Then, 2 ml of the solution was poured into beakers and the pH meter was placed in. The pH of each sample was measured in triplicate and the mean of the data was recorded.
Determination of titrable acidity
Maguire method was used to determine TA. 0.1 M sodium hydroxide (NaOH) droplets were added to the sample by a bort to reach a pH of 5.5 in pH meter. Each sample was titrated 3 times with this method, and the average amount of sodium hydroxide was recorded. The data obtained to calculate the TA are in the following formula:
TA = 0.1× (α/2 × 5 mL)
α = volume of sodium hydroxide used to reach pH 5.5/ml
5 mL = standard volume for TA calculation
0.1 = sodium hydroxide molarity
Since 2 ml of the sample is used, α is divided into 2.
Solutions with TA <0.5 are placed in the low-erosion group, TAs in the range of 0.5–2.5 are placed in the moderate erosion group, and TAs >2.5 are placed in the high-erosion group.
We used sample size of 30 permanent teeth (premolar) and 30 primary teeth which selected based on previous study  that were extracted due to orthodontics. The teeth were examined by a stereomicroscope, and the nonhypoplastic, Nonhypocalcification and nonfractioned teeth were selected. The teeth were washed to remove the blood, saliva, and other debris, and they were then cleaned with slurry of pumice and placed in the physiologic serum until the start of the experiment. Subsequently, the samples were fixed to the acrylic resin so that enamel appears. Then, to create a smooth surface, the outer enamel surface of specimens was ground with sandpaper (600 grit and then 1200 grit). Then, the microhardness of the enamel in all specimens was measured with Vickers hardness tester (MHZ, Koopa Company, Mashhad, Iran) using Vickers diamond indenter with 50 g load for 10 s. For each specimen, three different hardness tests were performed and the mean degree was reported. Subsequently, the groups were divided as follows:
- Primary tooth (D)–permanent teeth (P)
- Popping chocolate (A)–strawberry popping candy (B)–orange popping candy (C).
These were titles as DA-DB-DC-PA-PB-PC. Each group included 10 teeth.
All specimens were exposed to a solution containing 5 g of popping chocolate/candy dissolved in 2 mL of saliva twice a day for 5 min in 5 days. After each exposure, specimens were washed into distilled water for 20 s and then immersed in the artificial saliva until the next stage of the test. Artificial saliva was changed daily. Subsequently, retests were carried out with the Vickers hardness test. For each specimen, hardness was measured in triplicate and the average was reported. Before the start of the experiment, one sample of each group was sent to atomic force microscopy (AFM) (Easyscan2 Flex) to evaluate surface roughness, and topography was prepared. After the experiment, topography was made from the same sample [Figure 2] and [Figure 3].
|Figure 2: Two-dimensional and three-dimensional atomic force microscopy images, respectively, from the DB, DA, DC group (left: Before exposure, right: After exposure)|
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|Figure 3: Two-dimensional and three-dimensional atomic force microscopy images, respectively, from the PB, PA, PC group (left: Before exposure, right: After exposure)|
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| Results|| |
The results of this study showed that there was a significant difference between the microhardness of the permanent and primary tooth enamel before and after the exposure to popping chocolate and candy (paired t-test, P < 0.001) [Table 2].
|Table 2: Comparison of the microhardness before and after effect of chocolate and candies in each group (n=10) (paired t-test)|
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The most significant effect on the microhardness of enamel in permanent and primary teeth was orange candy (C), strawberry candy (B), and chocolate (A), respectively. This difference was significant (P< 0.05) (ANOVA) [Table 3].
|Table 3: Comparison of effect of candy and chocolate on the microhardness of the permanent and primary teeth enamel|
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The results of Tukey test showed that there is a significant difference between effects of chocolate and candy in both permanent teeth and primary teeth, but there is no significant difference between effects of the candies [Table 3].
The results of independent t-test showed that there was not a significant difference between the mean of microhardness with the effect of popping chocolate (A) (P = 0.89), strawberry popping candy (B) (0.48), and orange popping candy (C) (P = 0.38) (0.48) in permanent and primary teeth [Table 3].
Only the pH of the candies (strawberry and orange flavor) reached the critical pH of enamel (5.5), and thus, only their TA was calculated [Table 4].
|Table 4: Findings on determining the pH and titrable acidity of candy and chocolate|
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The findings from AFM are as follows:
The results of AFM have shown that Ra (index showing the surface roughness) has increased in all groups. Hence, surface roughness has increased [Figure 1].
| Discussion|| |
Different studies on the prevalence of dental erosion in children and adolescents have shown increased risk of teeth erosion with frequent use of candy. The aim of this study was to evaluate the effect of two types of popping candy and popping chocolate: orange Kick pop candy, strawberry Kick pop candy and Merdas popping chocolate on the microhardness of extracted human permanent and primary enamel tooth. The results showed that only the pH of the candies was below the critical pH of enamel, and in all groups, surface roughness has increased and also showed a reduction in the hardness of the enamel tooth after exposed to solutions containing candies or chocolate.
Hardness is, in fact, the resistance of a substance or surface to the indentation or penetration, which is an important mechanical property for matter. The material's resistance to wear or friction or erosion with water or any substances generally increases with hardness. This means that the enamel with more mineral content has less wear than dentine. Regarding the fact that, due to the exposure of the tooth structure to acidic solutions, first, the microhardness of the tooth structure decreases, and then, the surface tissue will be lost as a part of the erosion process. Therefore, we measured the permanent and primary enamel teeth hardness by vickers after their were effected by candy and chocolate. Hardness testing with Vickers is a standard method for evaluating the hardness of materials, especially hard materials, nonhomogeneous and prone to cracking like tooth enamel.
In previous studies, hardness testing was carried out by a Vickers hardness test device for each sample applying 50 g load for 10 s. In this study, 50 g load was applied for 10 s to determine the hardness of the teeth before and after the exposure. The results of this study showed that there was a significant difference between the two groups in the pre- and postexposure to chocolate and candies with different flavors, i.e. the microhardness of enamel after chocolate and candy consumption decreased, which is in line with the study of Mudumba et al. and Bolan et al. and Wagoner et al. illustrating the effects of candy and chocolate on reducing the acidity of the saliva and consequently the erosion of the enamel.
It has been shown in some studies that permanent enamel is more resistant to erosion compared to primary enamel., Hunter et al. showed that there was a significant difference between primary enamel and permanent enamel teeth after 15 days of orange juice consumption, but in this study, the effect of these chocolates and candies did not show significant difference in reducing the microhardness between permanent and primary teeth, which is also in line with the results of Bolan et al., Furthermore, Carvalho et al. studied the effects of nine different dietary substances on permanent and primary teeth enamel and surface hardness of the teeth after their immersion for 2 and 4 min; it was shown that there was no significant difference between the reduction of hardness in permanent and primary teeth after immersion.
pH and TA affect the potential of the erosive material. In this study, the pH of the orange candy (pH = 5.33) and strawberry candy (pH = 5.36) is lower than the critical pH of the enamel, which is 5.5, and their pH was also lower than the pH of the chocolate (pH = 6.30). In Lazzaris et al. studies, in 2015, when they examined the erosive potential of commercially available candies and their acidity, they also determined that the pH of the candies with different flavors varies, and the pH of strawberry candy, cherry candy, and orange candy was below the critical pH of the enamel. Furthermore, in the study of Leelavathi and Chaly, the pH of the lollipops with different flavors was measured, which is consistent with this study. In this study, candy and chocolate exposure to the teeth and it was shown that the reduction of microhardness was higher in orange candy, strawberry candy, and chocolate, respectively. Although the pH of the chocolate did not reach the critical pH of the enamel, it decreased its microhardness because, in addition to the pH, frequency, the time of exposure, the type, and concentration of acid affect on erosion.
In this study, as some studies , imitate the saliva-induced oral environment, artificial saliva was used as control group and intermediate medium. In the Davari et al. studies, the physiological serum was used. In Leelavathi studies, the volume of 10 mL was used to dissolve the candy, but in this study, according to the amount of stimulate saliva released per minute, which was 1–2 mL 13, the solution was considered as a 2 mL.
Different studies applied different regimens to immerse the samples in their investigated solutions.
The longer exposure time with acid has been reported 10–60 min and the shorter exposure time in range 1–4 min, so, given that most of the studies considered 5 min of exposure in the conditions of in vitro, specimens in our study, following Bolan et al., were exposed to acid for 5 min.
In dental erosion, enamel crystals are dissolved layer-to-layer. Therefore, to understand the early stages of the dissolution in enamel, it is necessary to precisely measure the mineral loss. In this study, AFM was used to determine the surface changes caused by the popping chocolate and candies. AFM gives images with high contrast and high resolution of the surface. In this study, one sample was selected from each group and the Ra (index showing the surface roughness) was reported and compared with Ra after exposure, which was consistent with the study of Poggio et al. in which after demineralization with an acidic substance such as Coca-Cola, there was an increase in surface roughness.
One of the limitations of this study was that only the permanent premolar teeth and primary incisors had been investigated. It is suggested that in later studies, the effect of these candies and chocolate should be performed on the extracted hidden teeth.
This study was also performed in vitro, and it is suggested that to construct closer conditions, studies should be performed as clinical examination in oral environment by measuring the pH of the saliva.
| Conclusions|| |
This study showed that orange Kick pop candy, strawberry Kick pop candy, and Merdas popping chocolate, especially orange Kick pop candy, reduce the microhardness of enamel and consequently lead to teeth erosion. Given the access of children and adolescents to the types of chocolates and candies examined in this study, it is suggested that families apply more control over children and adolescents for their consumption. Furthermore, it is suggested that they wash their mouth with water after taking the candies and chocolates. Toothbrushing is not immediately recommended because the enamel softened by acid is easily removed using toothbrush and toothpaste.
The present study was supported financially by the Deputy of Research of Babol University of Medical Sciences based on a research plan under the code(9643912). The author are gratefull to Babol University of Medical Sciences for providing the product and Babol Noshirvani University of Technology for AFM use.
Financial support and sponsorship
The study was financially supported by the Deputy of Research of Babol University of Medical Sciences.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Fejerskov O, Kidd E. Dental Caries: The Disease and Its Clinical Management. 2nd
ed.. Gray publishing, Tunbridge wells, UK: Wiley; 2009. p. 234.
Ispas A, Craciun A, Negucioiu M, Popa D, Lascu L, Constantiniuc M. The degree of involvement of etiological factors in different types of non-carious lesions. Hum Vet Med 2016;8:2016;8:119-123.
Monika G, Pandit IK, Nikhil S, Neeraj G. Dental erosion in children. J Oral Health Community Dent 2009;3:56-61.
Jain AA, Bhat M, Killada J, Yadhav OS, Singh I. Dental implications and laboratory evaluation of tooth dissolution in medicated liquid syrups. J Adv Med Dent Sci Res 2016;4:26.
Wang YL, Chang CC, Chi CW, Chang HH, Chiang YC, Chuang YC, et al.
Erosive potential of soft drinks on human enamel: An in vitro
study. J Formos Med Assoc 2014;113:850-6.
Bhowmick GS. Etiological and predisposing factors for dentin hypersensitivity: An overview. IJSS 2017;3:16.
Mudumba VL, Muppa R, Srinivas N, Kumar DM. Evaluation and comparison of changes in microhardness of primary and permanent enamel on exposure to acidic center-filled chewing gum: An in vitro
study. Int J Clin Pediatr Dent 2014;7:24-9.
Torres CP, Chinelatti MA, Gomes-Silva JM, Rizóli FA, Oliveira MA, Palma-Dibb RG, et al.
Surface and subsurface erosion of primary enamel by acid beverages over time. Braz Dent J 2010;21:337-45.
Leelavathi L, Chaly PE. Erosive potential of various commercially available lollipops. J Oral Health Community Dent 2015;9:126-31.
Lazzaris M, Gouveia Farias MM, Araújo SM, Schmitt BH, Silveira EG. Erosive potential of commercially available candies. Braz Res Pediatr Dent Integr Clin 2015;15:7-12.
Kleiner F, Roy PK, Kuchman MJ. Process of Preparing Gasified Candy, U.S. Patent 6,129,513; 15 September, 1981.
Davies R, Hunter L, Loyn T, Rees J. Sour sweets: A new type of erosive challenge? Br Dent J 2008;204:E3.
Mcdonald RE, Avery DR, Dean JA. Dentistry for the Child and Adolescent. 8th
ed. the university of michigan Mosby; 2004. p. 213.
Pasdar N, Qujeq D, Abolghasemzadeh F. Rate of PH and acidity titration of oral liquid drugs with long-term use in children. Iran J Pediatr Dent 2010;6:50.
Bolan M, Ferreira MC, Vieira RS. Erosive effects of acidic center-filled chewing gum on primary and permanent enamel. J Indian Soc Pedod Prev Dent 2008;26:149-52.
] [Full text]
Aljawad A, Morgan MZ, Rees JS, Fairchild R. The availability of novelty sweets within high school localities. Br Dent J 2016;220:575-9.
Arslan S, Zorba YO, Atalay MA, Özcan S, Demirbuga S, Pala K, et al.
Effect of resin infiltration on enamel surface properties and Streptococcus mutans
adhesion to artificial enamel lesions. Dent Mater J 2015;34:25-30.
Haghgoo R, Foruzesh Tabar F. The effect of carbonate beverage and soft beer on enamel micro-hardness of permanent teeth. J Dent Shiraz Univ Med Sci 2010;11:154-60.
Wagoner SN, Marshall TA, Qian F, Wefel JS.In vitro
enamel erosion associated with commercially available original-flavor and sour versions of candies. J Am Dent Assoc 2009;140:906-13.
Haghgou HR, Haghgoo R, Asdollah FM. Comparison of the microhardness of primary and permanent teeth after immersion in two types of carbonated beverages. J Int Soc Prev Community Dent 2016;6:344-8.
Zheng L, Peng J, Zheng J, Liu D, Zhou Z. Surface properties of eroded human primary and permanent enamel and the possible remineralization influence of CPP-ACP. Wear 2017;376:251-8.
Hunter ML, West NX, Hughes JA, Newcombe RG, Addy M. Erosion of deciduous and permanent dental hard tissue in the oral environment. J Dent 2000;28:257-63.
Carvalho TS, Schmid TM, Baumann T, Lussi A. Erosive effect of different dietary substances on deciduous and permanent teeth. Clin Oral Investig 2017;21:1519-26.
Gouveia Farias MM, de Oliveira MM, Eger Schmitt BH, da Silveira EG, de Araújo SM. Erosive potential of sugar-free hard candies dissolved in water and artificial saliva. Braz J Oral Sci 2016;15:75-78.
Vartanian LR, Schwartz MB, Brownell KD. Effects of soft drink consumption on nutrition and health: A systematic review and meta-analysis. Am J Public Health 2007;97:667-75.
Shenkin JD, Heller KE, Warren JJ, Marshall TA. Soft drink consumption and caries risk in children and adolescents. Gen Dent 2003;51:30-6.
Davari AR, Daneshkazemi AR, Farahat F, Zamani F. The evaluation of the effect of some common carbonated beverages in Iran on enamel microhardness of the human extracted teeth. Jundishapur Sci Med J 2016;15:607-17.
Gupta R, Solanki A, Sharma S, Gumber P, Sharma A, Upadhyay R. A knowledge, attitude and practices of soft drinks among adolescent students and their dental health: A Questionnaire study. Int J Dent Health Concern 2015;1:8-12.
Lussi A, Schlueter N, Rakhmatullina E, Ganss C. Dental erosion – An overview with emphasis on chemical and histopathological aspects. Caries Res 2011;45 Suppl 1:2-12.
Kashkosh L, Genaid T, Etman W. Effect of remineralization on metrology of surface features of induced acid eroded tooth enamel. Dent J 2016;62:514.
Poggio C, Ceci M, Beltrami R, Lombardini M, Colombo M. Atomic force microscopy study of enamel remineralization. Ann Stomatol (Roma) 2014;5:98-102.
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4]
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