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ORIGINAL ARTICLE
Year : 2014  |  Volume : 4  |  Issue : 2  |  Page : 92-95

In vitro evaluation of antimicrobial activity of different Gutta‑percha points and calcium hydroxide pastes


1 Department of Conservative Dentistry and Endodontics, Yamuna Institute of Dental Sciences and Research, Gadholi, Yamunanagar, Haryana, India
2 Department of Conservative Dentistry and Endodontics, KLE Dental College, Belgaum, Karnataka, India
3 Department of Conservative Dentistry and Endodontics, Indraprastha Dental College, Ghaziyabad, Uttar Pradesh, India
4 Department of Periodontology, Bhabha College of Dental Science, Bhopal, Madya Pradesh, India
5 Department of Oral and Maxillofacial Pathology, DJ College of Dental Sciences and Research, Modinagar, Uttar Pradesh, India

Correspondence Address:
Ashu Jhamb
Department of Conservative Dentistry and Endodontics, Yamuna Institute of Dental Sciences and Research, Gadholi, Yamuna Nagar - 135 001, Haryana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-0762.137648

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Purpose: To evaluate the antimicrobial activity of different compositions of Gutta‑percha points and calcium hydroxide (Ca(OH)2) pastes, used in endodontic therapy. Materials and Methods: The evaluated material consisted of Gutta‑percha points containing Ca(OH)2, Gutta‑percha points containing chlorhexidine (Chx), conventional Gutta-percha points and Ca(OH)2 pastes. Antimicrobial properties of Chx and CaOH paste are compared with CaOH points. Antimicrobial tests included three species of microorganisms: Escherichia coli (ATCC 25923), Staphylococcus aureus (ATCC 25922) Pseudomonas aeruginosa (ATCC BAA‑427), the agar disc diffusion method was employed. The plates were kept at room temperature for 2 h for prediffusion and then incubated at 37΀C for 24 h. Zones of inhibition were measured. Results and Conclusion: All microbial species used in the study were inhibited by the Gutta‑percha points containing Chx and by the Ca(OH)2 pastes, no antimicrobial activity was observed for the other groups (conventional Gutta‑percha and Ca(OH)2 group).


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