|Year : 2020 | Volume
| Issue : 4 | Page : 491-497
|Clinical and microbiological profiles of aggressive and chronic periodontitis in Congolese patients: A cross-sectional study
Em Kalala-Kazadi1, Selena Toma2, Jérôme F Lasserre2, Fidèle Nyimi-Bushabu3, Hubert Ntumba-Mulumba4, Michel C Brecx2
1 Periodontology Unit, Department of Dental Medicine, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
2 Department of Periodontology, Université Catholique de Louvain, Brussels, Belgium
3 Oral and maxillo-facial surgery, Department of Dental Medicine, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
4 Prosthodontics and Orthodontics Service, Department of Dental Medicine, Faculty of Medicine, University of Kinshasa, Kinshasa, Democratic Republic of Congo
|Date of Submission||17-Dec-2019|
|Date of Decision||02-Feb-2020|
|Date of Acceptance||17-Feb-2020|
|Date of Web Publication||06-Aug-2020|
Dr. Em Kalala-Kazadi
Periodontology Unit, Department of Dental Medicine, University of Kinshasa, B.P. 834 Kinshasa 11.
Democratic Republic of Congo
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Chronic and aggressive periodontitis were the main forms of periodontitis according to the 1999 classification of periodontal diseases and conditions. Their profile in Congolese patients is still undescribed. Aim: The aim of this study was to compare the profile of chronic periodontitis (ChP) with that of aggressive periodontitis (AgP) in Congolese patients. Materials and Methods: Thirty-two patients with ChP and 20 with AgP who consulted the dental services at any of the four medical centers in Kinshasa, from April 2017 to April 2018, were enrolled in the cross-sectional study. All patients underwent a full mouth examination, including assessment of the probing pocket depth and clinical attachment level at six sites per tooth. Microbial samples were collected in the deepest pocket in the maxilla and the deepest pocket in the mandible. A deoxyribonucleic acid (DNA) analysis was performed using DNA strip technology. Fisher exact test, the chi-square test, the t test, and the Mann–Whitney test were used for the statistical analysis. Results: Patients with AgP were significantly younger than those with ChP (P < 0.001). There was no significant difference in the prevalence of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, or Prevotella intermedia between the AgP and ChP groups (P > 0.05). Aggregatibacter actinomycetemcomitans was detected in 10% of cases in the AgP group and in none of those in the ChP group (P = 0.143). Conclusion: This study shows that the clinical profiles of ChP and AgP are similar in Congolese patients. There were no microbiological differences between these two forms of periodontitis.
Keywords: Aggressive periodontitis, bacteria, chronic periodontitis, microbiological profile, periodontitis
|How to cite this article:|
Kalala-Kazadi E, Toma S, Lasserre JF, Nyimi-Bushabu F, Ntumba-Mulumba H, Brecx MC. Clinical and microbiological profiles of aggressive and chronic periodontitis in Congolese patients: A cross-sectional study. J Int Soc Prevent Communit Dent 2020;10:491-7
|How to cite this URL:|
Kalala-Kazadi E, Toma S, Lasserre JF, Nyimi-Bushabu F, Ntumba-Mulumba H, Brecx MC. Clinical and microbiological profiles of aggressive and chronic periodontitis in Congolese patients: A cross-sectional study. J Int Soc Prevent Communit Dent [serial online] 2020 [cited 2020 Nov 25];10:491-7. Available from: https://www.jispcd.org/text.asp?2020/10/4/491/291464
| Introduction|| |
Periodontitis is an inflammatory/biofilm-induced disease characterized by loss of the tissues supporting the teeth. Microbes within the dental biofilm initiate inflammation that can lead to breakdown of tissues in a susceptible host.
According to the 1999 classification of periodontal diseases and conditions, three primary forms of periodontitis are distinguished, of which aggressive periodontitis (AgP) and chronic periodontitis (ChP) are the most common. The difference between these two forms of periodontitis has been a matter of intense debate for the past two decades, and studies distinguishing these forms are being published nowadays.,,,,,, According to a recent workshop on the classification of periodontal and peri-implant diseases and conditions, there is currently insufficient evidence to consider AgP and ChP as two pathobiologically distinct diseases. This has led to patients with the chronic and aggressive forms being grouped under a single category of “periodontitis,” implying that research should continue to provide scientific evidence that can support or refute this framework. Fine et al. have recently concluded that localized AgP differs in many ways from the far more common ChP and supported classifying it as a distinct form of periodontitis. Furthermore, comparing these two forms of periodontitis in Africa, where this disease is reported to be very common, may be of some interest.
The microbiota associated with periodontitis seems to vary markedly according to geographic location and between different ethnic or racial groups.,, According to Mayorga-Fayad et al., the evidence suggests that each country must establish its own oral microbiological profile to formulate adequate measures and more specific treatments.
Various studies have attempted to determine biological, clinical, and microbiological differences between AgP and ChP to facilitate the diagnosis and treatment of periodontal disease. A systematic review by Mombelli et al. concluded that the presence or absence of the periopathogens assessed in their study could not discriminate between subjects with AgP and those with ChP. Another study analyzed the prevalence and levels of 51 species by checkerboard DNA-DNA hybridization and found few differences in microbial species between AgP and ChP but that Porphyromonas gingivalis and Treponema denticola were associated with ChP.
Acquisition of additional microbial data at the population level would contribute to better clinical diagnosis and a better treatment plan.,,
The objective of this study was to compare the clinical and microbiological profiles of AgP and ChP in Congolese patients.
| Materials and Methods|| |
The subjects of this cross-sectional study were selected from patients who attended for dental services at any of the four medical centers in Kinshasa, that is, the Biamba Marie Mutombo Hospital, University Clinic, Ngaliema Clinic, or Boyambi Clinic, between April 2017 and April 2018. The study protocol was approved by the ethics committee at the School of Public Health, University of Kinshasa (approval number ESP/015/2017) and conducted according to the tenets outlined in the Declaration of Helsinki and to the STROBE guidelines.
Selection of study population and clinical examination
The study included 52 patients (18 men and 34 women) of mean age 44 ± 18.5 (range, 14–72) years. Twenty patients had AgP and 32 had ChP. AgP and ChP were defined according to the criteria published by the American Academy of Periodontology. The mean age was 25.2 ± 11.9 years in the AgP group and 55.7 ± 10.5 years in the ChP group. The sample size was determined according to the method described in [Figure 1].
|Figure 1: Flowchart of study sample. AgP = aggressive periodontitis, CAL = clinical attachment level, ChP = chronic periodontitis, PPD = probing pocket depth|
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The study inclusion criteria were as follows: at least 20 teeth present, age 12 years or older, a probing pocket depth (PPD) of at least 5 mm and a clinical attachment level (CAL) of 3 mm for at least three teeth (at least one in the maxilla and another in the mandible), and a diagnosis of AgP or ChP. For AgP, at least two of the teeth involved were required be a first molar or incisor. Patients who had taken antibiotics during the previous 6 months and those were receiving or had received periodontal treatment during the previous 6 months were excluded, as were patients who were pregnant or lactating.
A full mouth examination was performed using a PCP 10 periodontal probe (Hu-Friedy, Chicago, Illinois). All teeth, except for the third molars, were examined. Teeth that were erupting, supernumerary, or partially impacted were also excluded. The PPD and CAL were assessed at six sites (mesiobuccal, buccal, distobuccal, mesiolingual, lingual, and distolingual) per tooth. The plaque index and bleeding on probing (BoP) were also recorded. All measurements were performed by the same trained examiner (EKK); 20 patients were examined and reexamined 1h later. The κ coefficients calculated for PPD and CAL were 0.82 and 0.84, respectively.
Subgingival biofilm samples were collected using a previously described method. They were obtained from the two deepest pockets per patient (one in the upper arch and another in the lower arch) determined during the clinical examination.
The sampled site was isolated using sterile cotton rolls. The supragingival plaque was first removed with sterile gauze and then a sterile Gracey curette. A sterile paper point was introduced into the pocket and left in place for 10s. The paper point was then removed and placed immediately in an Eppendorf plastic tube. The tube was then closed and placed in the micro-IDent kit provided by Biocentric Laboratory (Hain Lifescience, Nehren, Germany).
The plaque samples were sent to France where polymerase chain reaction (PCR) analysis was performed by the Biocentric Laboratory (Bandol, France). The technique used in this laboratory is based on semiquantitative PCR using DNA-strip technology (Hain Lifescience). The threshold for detection of bacteria is 10³ for Aggregatibacter actinomycetemcomitans and 104 for P. gingivalis, Tannerella forsythia, T. denticola, and Prevotella intermedia. DNA was extracted from the specimen by thermolysis at 95°C. The DNA was selectively amplified by subsequent PCR. The amplified DNA was denaturized and then hybridized with specific probes on strip.
The statistical analysis was performed using Statistical Package for Social Sciences software, version 20.0 (IBM, Armonk, New York). Comparisons between AgP and ChP were performed using Fisher exact test, the t test, the chi-square test, and the Mann–Whitney test.
| Results|| |
The mean patient age was significantly lower in the AgP group than that in the ChP group (25.2 ± 11.9 years vs. 55.7 ± 10.5 years; P < 0.001). Furthermore, there was no significant difference in patient sex or educational level between the AgP and ChP groups (P = 0.065 and P = 0.375, respectively). All current smokers had ChP but there was no difference in smoking status between the two study groups (P = 0.151) [Table 1]. There was also no difference in the plaque index or number of missing teeth between the two clinical forms of periodontitis. BoP was greater in the AgP group than that in the ChP group (P = 0.025) [Table 2].
|Table 1: Demographic/lifestyle characteristics of patients with aggressive and chronic periodontitis|
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|Table 2: Clinical characteristics of aggressive and chronic periodontitis|
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[Table 3] compares the AgP and ChP groups according to the presence or absence of periopathogens, that is, P. gingivalis, T. forsythia, T. denticola, and P. intermedia, and found no significant difference in their distribution in the plaque samples (P > 0.05). A. actinomycetemcomitans was detected in 10% of cases in the AgP and in none of those in the ChP group (P = 0.143).
|Table 3: Comparison between aggressive periodontitis and chronic periodontitis according to the absence or presence of periopathogens|
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| Discussion|| |
The objective of this study was to compare the clinical and microbiological profiles of AgP and ChP in Congolese patients. Its findings indicated a marked difference in patient age between the AgP group and the ChP group but that none of the five types of bacteria assessed could differentiate the two forms of periodontitis by its presence or absence.
Age was the main demographic characteristic that distinguished ChP from AgP. The 1989 classification used age-dependent terms, such as “adult” and “juvenile,” to categorize periodontitis. The 1999 classification estimated that age was not an appropriate parameter for distinguishing the different forms of the disease and recommended its deletion., However, in daily practice and in epidemiological studies, age has continued to be an essential criterion for distinguishing between AgP and ChP., A pilot study that tested the consistency of diagnosis of AgP and ChP by certified periodontists found that awareness of patient age influenced the clinical diagnosis when a distinction was made between AgP and ChP.
Our results suggest that patients who have consulted a dentist previously are less likely to develop AgP than ChP. Given that AgP is considered to be a severe type of periodontitis, it is likely that Congolese patients who have sought dental care would be protected from a severe form of the disease.
The distribution of clinical features between the two types of periodontitis was slightly different; BoP in particular was greater in the AgP group than that in the ChP group. BoP has been reported to be a sign of gingival inflammation and a possible sign of progression of the disease, whereas its absence is a reliable predictor of periodontal health., The higher BoP in the AgP group may reflect the rapid progression of this severe form of periodontitis. The differences in PPD and CAL found in this study seem to be nonconclusive.
A. actinomycetemcomitans, reported to be a key pathogen in AgP,, was detected at low prevalence and only in the AgP group in this study. This finding is in agreement with other studies in which this organism was detected at low prevalence., Other researchers have also reported an absence of A. actinomycetemcomitans in patients with AgP. Variations according to geographic location and ethnic or racial group could explain the low prevalence of A. actinomycetemcomitans in Congolese patients diagnosed clinically with AgP. The other bacteria assessed in this study, that is, P. gingivalis, T. forsythia, and T. denticola, were all detected at high prevalence. This is in agreement with our previous pilot study in which red complex bacteria were present at high prevalence.
No statistically significant difference in periopathogen status was found between our ChP and AgP groups. The presence of P. gingivalis, T. forsythia, T. denticola, P. intermedia, and A. actinomycetemcomitans could not differentiate between the two forms of periodontal disease. A systematic review by Mombelli et al. concluded that the presence or absence of P. gingivalis, A. actinomycetemcomitans, P. intermedia, T. forsythia, and Campylobacter rectus could not discriminate between subjects with AgP and those with ChP. Other studies have not found a microbiological difference between these two forms of periodontal disease., Another study found no differences in the immunological and microbiological profiles of ChP and AgP and concluded that these two clinically distinct forms of periodontitis might result from common pathogenic mechanisms.
Within the limitations of this study, ChP and AgP seem quite similar in Congolese patients except for clinical periodontal inflammation that was more visible in AgP. No microbiological difference was found between these two forms of periodontitis. However, given the low power of the tests used to compare microbiological profile, studies with great samples are needed in Congolese patients. Age remained an essential factor for differentiation between AgP and ChP.
We would like to thank Dr. Marc Tordjeman for helping with the analysis of the plaque samples, and Professor Magda Feres for assistance with elaboration of the protocol regarding the subgingival dental plaque sampling method. We would also like to thank Editage (www.editage.com) for English language editing.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
Concepts: Em Kalala-Kazadi, Michel C. Brecx, Hubert Ntumba-Mulumba.
Design: Em Kalala-Kazadi, Michel C. Brecx, Hubert Ntumba-Mulumba.
Definition of intellectual content: Em Kalala-Kazadi, Fidèle Nyimi-Bushabu, Michel C. Brecx.
Literature search: Em Kalala-Kazadi, Selena Toma.
Clinical studies: Em Kalala-Kazadi.
Data acquisition: Em Kalala-Kazadi.
Data analysis: Em Kalala-Kazadi, Selena Toma, Jérôme F. Lasserre, Michel C. Brecx.
Statistical analysis: Em Kalala-Kazadi, Selena Toma, Jérôme F. Lasserre.
Manuscript preparation: Em Kalala-Kazadi, Fidèle Nyimi-Bushabu, Hubert Ntumba-Mulumba.
Manuscript editing: Em Kalala-Kazadi, Selena Toma, Jérôme F. Lasserre, Michel C. Brecx.
Manuscript review: Em Kalala-Kazadi, Selena Toma, Jérôme F. Lasserre, Fidèle Nyimi-Bushabu, Hubert Ntumba-Mulumba, Michel C. Brecx.
Guarantor: Michel C. Brecx.
Ethical policy and institutional review board statement
The study protocol was approved by the ethics committee at the School of Public Health, University of Kinshasa (approval number, ESP/015/2017) and conducted according to the tenets outlined in the Declaration of Helsinki.
Patient declaration of consent
Data availability statement
The data set used in this study is available on request from Dr. Em Kalala-Kazadi ([email protected]).
| References|| |
Yost S, Duran-Pinedo AE, Teles R, Krishnan K, Frias-Lopez J Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. Genome Med 2015;7:27.
Armitage GC Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999;4:1-6.
Schmidt J, Jentsch H, Stingu CS, Sack U General immune status and oral microbiology in patients with different forms of periodontitis and healthy control subjects. PLoS One 2014;9:e109187.
Teodorescu AC, Martu I, Teslaru S, Kappenberg-Nitescu DC, Goriuc A, Luchian I, et al
. Assessment of salivary levels of RANKL and OPG in aggressive versus chronic periodontitis. J Immunol Res 2019;2019:6195258.
Wei Y, Shi M, Zhen M, Wang C, Hu W, Nie Y, et al
. Comparison of subgingival and buccal mucosa microbiome in chronic and aggressive periodontitis: A pilot study. Front Cell Infect Microbiol 2019;9:53.
Schulz S, Porsch M, Grosse I, Hoffmann K, Schaller HG, Reichert S Comparison of the oral microbiome of patients with generalized aggressive periodontitis and periodontitis-free subjects. Arch Oral Biol 2019;99:169-76.
Amaral SA, Pereira TSF, Brito JAR, Cortelli SC, Cortelli JR, Gomez RS, et al
. Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis. Oral Dis 2019;25:561-8.
Baltacioglu E, Sukuroglu E Protein carbonyl levels in serum, saliva and gingival crevicular fluid in patients with chronic and aggressive periodontitis. Saudi Dent J 2019;31:23-30.
Masumoto R, Kitagaki J, Fujihara C, Matsumoto M, Miyauchi S, Asano Y, et al
. Identification of genetic risk factors of aggressive periodontitis using genomewide association studies in association with those of chronic periodontitis. J Periodontal Res 2019;54:199-206.
Jamesha FI, Maradi AP, Chithresan K, Janakiram S, Maddur PK, Rangaraju R Comparison of gingival crevicular fluid periostin levels in healthy, chronic periodontitis, and aggressive periodontitis. J Indian Soc Periodontol 2018;22:480-6.
Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al
. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions. J Periodontol 2018;89:S173-82.
Caston JG, Armitage G, Berglundh T, Chapple ILC, Jepsen S, Kornman KS, et al
. A new classification scheme for periodontal and peri-implant diseases and conditions—Introduction and key changes from the 1999 classification. J Clin Periodontol 2018;45:S1-8.
Fine DH, Armitage GC, Genco RJ, Griffen AL, Diehl SR Unique etiologic, demographic, and pathologic characteristics of localized aggressive periodontitis support classification as a distinct subcategory of periodontitis. J Am Dent Assoc 2019;150:922-31.
Feng X, Zhang L, Xu L, Meng H, Lu R, Chen Z, et al
. Detection of eight periodontal microorganisms and distribution of Porphyromonas gingivalis
Fima genotypes in Chinese patients with aggressive periodontitis. J Periodontol 2014;85: 150-9.
Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, et al
. Subgingival microbial profiles in chronic periodontitis patients from Chile, Colombia and Spain. J Clin Periodontol 2008;35:106-13.
Kim TS, Kang NW, Lee SB, Eickholz P, Pretzl B, Kim CK Differences in subgingival microflora of Korean and German periodontal patients. Arch Oral Biol 2009;54:223-9.
Kalala-Kazadi E, Sekele-Issouradi JP, Bolenge-Ileboso J, Lasserre JF, Mantshumba-Milolo A, Ntumba-Mulumba H, et al
. Periopathogenic bacteria in dental plaque of Congolese patients with periodontitis: A pilot study. J Clin Exp Dent 2018;10:e232-6.
Mayorga-Fayad I, Lafaurie GI, Contreras A, Castillo DM, Barón A, Aya Mdel R [Subgingival microbiota in chronic and aggressive periodontitis in Bogotá, Colombia: An epidemiological approach]. Biomedica 2007;27:21-33.
Mombelli A, Casagni F, Madianos PN Can presence or absence of periodontal pathogens distinguish between subjects with chronic and aggressive periodontitis? A systematic review. J Clin Periodontol 2002;29:10-21; discussion 37-8.
Heller D, Silva-Boghossian CM, do Souto RM, Colombo AP Subgingival microbial profiles of generalized aggressive and chronic periodontal diseases. Arch Oral Biol 2012;57:973-80.
Shaddox LM, Walker C Microbial testing in periodontics: Value, limitations and future directions. Periodontol 2000 2009;50:25-38.
Listgarten MA, Loomer PM Microbial identification in the management of periodontal diseases. A systematic review. Ann Periodontol 2003;8:182-92.
Eick S, Pfister W Comparison of microbial cultivation and a commercial PCR based method for detection of periodontopathogenic species in subgingival plaque samples. J Clin Periodontol 2002;29:638-44.
Armitage GC Comparison of the microbiological features of chronic and aggressive periodontitis. Periodontol 2000 2010;53:70-88.
Demmer RT, Papapanou PN Epidemiologic patterns of chronic and aggressive periodontitis. Periodontol 2000 2010;53:28-44.
Armitage GC, Cullinan MP Comparison of the clinical features of chronic and aggressive periodontitis. Periodontol 2000 2010;53:12-27.
Oshman S, El Chaar E, Lee YN, Engebretson S Effect of patient age awareness on diagnostic agreement of chronic or aggressive periodontitis between clinicians; a pilot study. BMC Oral Health 2016;17:27.
Lang NP, Joss A, Orsanic T, Gusberti FA, Siegrist BE Bleeding on probing. A predictor for the progression of periodontal disease? J Clin Periodontol 1986;13:590-6.
Lang NP, Bartold PM Periodontal health. J Periodontol 2018;89:S9-16.
Schacher B, Baron F, Rossberg M, Wohlfeil M, Arndt R, Eickholz P Aggregatibacter actinomycetemcomitans
as indicator for aggressive periodontitis by two analysing strategies. J Clin Periodontol 2007;34:566-73.
Elamin A, Albandar JM, Poulsen K, Ali RW, Bakken V Prevalence of Aggregatibacter actinomycetemcomitans
in Sudanese patients with aggressive periodontitis: A case-control study. J Periodontal Res 2011;46:285-91.
Takeuchi Y, Umeda M, Ishizuka M, Huang Y, Ishikawa I Prevalence of periodontopathic bacteria in aggressive periodontitis patients in a Japanese population. J Periodontol 2003;74:1460-9.
Faveri M, Mayer MP, Feres M, de Figueiredo LC, Dewhirst FE, Paster BJ Microbiological diversity of generalized aggressive periodontitis by 16S rRNA clonal analysis. Oral Microbiol Immunol 2008;23:112-8.
Benrachadi L, Bouziane A, Azziman Z, Bouziane-Ouartini F, Ennibi O Screening for periodontopathogenic bacteria in severe chronic periodontitis in a Moroccan population. Med Mal Infect 2012;42:599-602.
Rescala B, Rosalem W Jr, Teles RP, Fischer RG, Haffajee AD, Socransky SS, et al
. Immunologic and microbiologic profiles of chronic and aggressive periodontitis subjects. J Periodontol 2010;81:1308-16.
[Table 1], [Table 2], [Table 3]